Structure and analysis of a complex between SUMO and Ubc9 illustrates features of a conserved E2-Ubl interaction Journal Article
| Authors: | Capili, A. D.; Lima, C. D. |
| Article Title: | Structure and analysis of a complex between SUMO and Ubc9 illustrates features of a conserved E2-Ubl interaction |
| Abstract: | The SUMO E2 Ubc9 serves as a lynchpin in the SUMO conjugation pathway, interacting with the SUMO E1 during activation, with thioester linked SUMO after E1 transfer and with the substrate and SUMO E3 ligases during conjugation. Here, we describe the structure determination of a non-covalent complex between human Ubc9 and SUMO-1 at 2.4 Å resolution. Non-covalent interactions between Ubc9 and SUMO are conserved in human and yeast insomuch as human Ubc9 interacts with each of the human SUMO isoforms, and yeast Ubc9 interacts with Smt3, the yeast SUMO ortholog. Structural comparisons reveal similarities to several other non-covalent complexes in the ubiquitin pathway, suggesting that the non-covalent Ubc9-SUMO interface may be important for poly-SUMO chain formation, for E2 recruitment to SUMO conjugated substrates, or for mediating E2 interactions with either E1 or E3 ligases. Biochemical analysis suggests that this surface is less important for E1 activation or di-SUMO-2 formation, but more important for E3 interactions and for poly-SUMO chain formation when the chain exceeds more than two SUMO proteins. © 2007 Elsevier Ltd. All rights reserved. |
| Keywords: | unclassified drug; mutation; nonhuman; ubiquitin; chemical analysis; protein analysis; complex formation; protein; protein binding; protein interaction; mutational analysis; amino acid sequence; molecular sequence data; sequence homology, amino acid; models, statistical; crystallography, x-ray; protein structure, tertiary; dna mutational analysis; yeast; protein structure; ubiquitin protein ligase e3; sumo-1 protein; ubiquitin-conjugating enzymes; isoprotein; protein isoforms; molecular conformation; sumo; smt3; ubc9; x-ray structure; x-rays; orthology; sumo 1 protein; ubiquitin-like; protein ubc9; glycoprotein e1; glycoprotein e2; smt 3 protein |
| Journal Title: | Journal of Molecular Biology |
| Volume: | 369 |
| Issue: | 3 |
| ISSN: | 0022-2836 |
| Publisher: | Academic Press Inc., Elsevier Science |
| Date Published: | 2007-06-08 |
| Start Page: | 608 |
| End Page: | 618 |
| Language: | English |
| DOI: | 10.1016/j.jmb.2007.04.006 |
| PUBMED: | 17466333 |
| PROVIDER: | scopus |
| PMCID: | PMC1940065 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 41" - "Export Date: 17 November 2011" - "CODEN: JMOBA" - "Source: Scopus" |
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