In vivo 5-fluorouracil and fluoronucleotide T1 relaxation time measurements using the variable nutation angle method Journal Article
| Authors: | Gade, T. P. F.; Spees, W. M.; Le, H. C.; Zakian, K. L.; Ponomarev, V.; Doubrovin, M.; Gelovani, J. G.; Koutcher, J. A. |
| Article Title: | In vivo 5-fluorouracil and fluoronucleotide T1 relaxation time measurements using the variable nutation angle method |
| Abstract: | 19Fluorine NMRS has the potential to enable noninvasive predictions of tumor response to 5-fluorouracil (5FU) therapy based on tumor pharmacokinetics. Knowledge of the T1's of 5FU and its fluoronucleotide anabolites (FNuc) is required for quantitative spectral analysis and selection of optimal pulse parameters. We used the variable nutation angle (VNA) method to determine T1's of 5FU and FNuc in subcutaneous Walker 256 rat mammary carcinosarcoma tumors transfected with a cytosine deaminase/uracil phosphoribosyltransferase fusion gene. We calibrated in vivo NAs using methoxydifluoroacetate to ensure the accuracy of these measurements. The T1's were calculated based on signal intensities acquired with NAs of 20°, 35°, 45°, 60°, and 75°. The acquisition order of these NAs was shuffled to reduce the effect of signal variations. The determined T1's for 5FU and FNuc (2.3 ± 0.1 s and 1.3 ± 0.1 s, respectively) represent the first reported in vivo measurements for these metabolites in tumor. © 2004 Wiley-Liss, Inc. |
| Keywords: | controlled study; unclassified drug; fluorouracil; nonhuman; linear models; animal cell; animals; mice; analytic method; calibration; animal experiment; animal model; in vivo study; tumor cells, cultured; transfection; prediction; genetic transfection; mice, nude; quantitative analysis; breast carcinoma; rat; measurement; magnetic resonance spectroscopy; nuclear magnetic resonance spectroscopy; rats; phantom; 5-fluorouracil; technique; in vivo; nucleotide; mammary neoplasms, experimental; carcinosarcoma; cytosine deaminase; uracil phosphoribosyltransferase; relaxation time; male; female; article; fluoronucleotides; t1 relaxation time; variable nutation angle; fluoronucleotide; variable nutation angle method |
| Journal Title: | Magnetic Resonance in Medicine |
| Volume: | 52 |
| Issue: | 1 |
| ISSN: | 0740-3194 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2004-07-01 |
| Start Page: | 169 |
| End Page: | 173 |
| Language: | English |
| DOI: | 10.1002/mrm.20131 |
| PROVIDER: | scopus |
| PUBMED: | 15236381 |
| DOI/URL: | |
| Notes: | Magn. Reson. Med. -- Cited By (since 1996):5 -- Export Date: 16 June 2014 -- CODEN: MRMEE -- Source: Scopus |
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