Delayed-late activation of a myeloid defensin minimal promoter by retinoids and inflammatory mediators Journal Article
| Authors: | Wang, N.; Su, Q.; Boeckh-Herwig, S.; Yaneva, M.; Tempst, P. |
| Article Title: | Delayed-late activation of a myeloid defensin minimal promoter by retinoids and inflammatory mediators |
| Abstract: | α-Defensin-1 gene expression in promyelocytic HL-60 cells is ('delayed-late' ≥1-2 days) activated by retinoic acid (RA), lipopolysaccharide, tumor necrosis factor-α and elevated levels of cAMP. Using stably integrated reporter constructs, we show that this activation is directed through a proximal and distal element within a minimal (-83/+82) def1 promoter, and is mediated by phosphorylation of the associated factors, PU.1 and D1BP, in an inducer-dependent manner. Whereas binding of PU.1 to the proximal element confers cell specificity and relays the effects of most inducers, the selectively enhancing capacity of the distal element for RA- and cAMP-dependent activation is uniquely correlated with D1BP-binding. We propose that D1BP and PU.1 are the end-points of separate pathways. © 2004 Elsevier Ltd. All rights reserved. |
| Keywords: | protein phosphorylation; human cell; promoter region; proto-oncogene proteins; sequence deletion; antineoplastic agents; gene expression; transcription initiation; protein binding; transcription, genetic; hela cells; phosphorylation; cell specificity; gene expression regulation, neoplastic; correlation analysis; tumor necrosis factor alpha; tumor necrosis factor-alpha; transcription; cyclic amp; lipopolysaccharide; binding sites; genes, reporter; trans-activators; bone marrow cell; cell activation; electrophoretic mobility shift assay; retinoic acid; regulatory sequences, nucleic acid; retinoids; autacoid; lipopolysaccharides; camp; promoter regions (genetics); granulocytes; hl-60 cells; cell strain hl 60; defensin; alpha defensin; retinoid derivative; humans; human; priority journal; article; alpha-defensins |
| Journal Title: | Leukemia Research |
| Volume: | 28 |
| Issue: | 8 |
| ISSN: | 0145-2126 |
| Publisher: | Elsevier Ltd |
| Date Published: | 2004-08-01 |
| Start Page: | 879 |
| End Page: | 889 |
| Language: | English |
| DOI: | 10.1016/j.leukres.2003.12.005 |
| PROVIDER: | scopus |
| PUBMED: | 15203286 |
| DOI/URL: | |
| Notes: | Leuk. Res. -- Cited By (since 1996):3 -- Export Date: 16 June 2014 -- CODEN: LERED -- Source: Scopus |
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