Retinoic acid stimulates protein kinase A-associated G proteins during human teratocarcinoma differentiation Journal Article
| Authors: | Kurie, J. M.; Allopenna, J.; Dmitrovsky, E. |
| Article Title: | Retinoic acid stimulates protein kinase A-associated G proteins during human teratocarcinoma differentiation |
| Abstract: | Retinoic acid (RA) treatment of F9 murine teratocarcinoma (TC) cells reduces the expression of the protein kinase A (PKA)-associated G protein, G(alpha i2). The present study reveals interactions between the RA and PKA pathways during differentiation of the multipotent human TC cell line NTERA-2 clone D1 (abbreviated NT2/D1) which differ from prior reports in F9 TC cells. Compared to untreated NT2/D1 cells, differentiated NT2/D1 cells expressed increased levels of G(alpha S) and G(alpha il,2) proteins as shown by both immunoblot analysis and cholera toxin- and pertussis toxin-induced ADP ribosylation. To further explore cooperation between these pathways during human TC differentiation, we examined the effects of cyclic adenosine monophosphate (cAMP) on RA-responsive genes and of RA treatment on the transcriptional activation of a cAMP response element (CRE). Compared to RA alone, combined treatment with RA and cAMP augmented the expression of the RA nuclear receptor-beta (RAR-beta). Also, transient transfection assays revealed that cAMP and RA cooperated to enhance CRE transcriptional activation. The cAMP-induced enhancement of RA actions in NT2/D1 cells extended to immunophenotypic changes typical of the neuronal differentiation program induced by RA. In contrast to these findings in NT2/D1 cells, prior work in F9 TC cells showed that cAMP inhibits the RA-mediated augmentation of RAR-beta expression and switches the differentiation program from visceral to parietal endoderm. Thus, unlike murine TC cells, in human NT2/D1 cells RA stimulates PKA-associated G proteins and PKA pathway activation enhances RA-mediated TC differentiation. |
| Keywords: | dna; transcription; cyclic amp; protein kinase a; nuclear receptors; retinoic acid; g protein; expression; embryonal carcinoma-cells; line; thyroid-hormone; ribonucleic-acid; x-receptor; rxr-beta; retinoic acid nuclear receptor; teratocarcinoma differentiation; (human) |
| Journal Title: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research |
| Volume: | 1222 |
| Issue: | 1 |
| ISSN: | 0167-4889 |
| Publisher: | Elsevier B.V. |
| Date Published: | 1994-05-26 |
| Start Page: | 88 |
| End Page: | 94 |
| Language: | English |
| ACCESSION: | WOS:A1994NP34000011 |
| DOI: | 10.1016/0167-4889(94)90028-0 |
| PROVIDER: | wos |
| PUBMED: | 8186270 |
| Notes: | Article -- Source: Wos |
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