| Abstract: |
Induced transformation of mouse fibroblasts was carried out by releasing tetracycline-repressed expression of an oncogenic mutant of STAT3, STAT3-C, or of v-Src or Ha-Ras. At 15 days after derepression of each oncogene, DNA microarrays showed elevation (> 3-fold) of a similar group of ∼25 mRNAs compared to untransformed cells. RT-PCR confirmed a number of these mRNA elevations. RNA samples were then analysed at intervals during the first 24 h after doxycycline removal to determine the time of early changes. Extensive changes were not observed by array analysis, except in v-Src-expressing cells where about 10 mRNAs were elevated threefold or more. However, RT-PCR did uncover changes in each derepressed cell type that included some of the changes observed after the 15-day transformation period. In addition, STAT3-C target genes such as BclXI and cyclin D1, which were not observed on array analysis, were elevated by RT-PCR analysis. We conclude, therefore, that early after oncogene induction, transcriptional changes, including those initiated by STAT3-C, may occur only in scarce mRNA and/or to a limited extent. However, with additional time and probably additional cell division, a new epigenetic state is established that is mirrored by a changed transcriptional profile emblematic of transformation by each of three oncogenes. |
| Notes: |
Oncogene -- Cited By (since 1996):22 -- Export Date: 16 June 2014 -- CODEN: ONCNE -- Molecular Sequence Numbers: GENBANK: AB006758, AB011030, AB013137, AB025413, AF013099, AF013262, AF017128, AF061260, AF099973, AJ002366, AJ002390, AJ249706, D30782, D76440, D86370, J03482, K02245, K03235, L02914, L12703, L29123, L32836, L47480, M20658, M33960, M60348, M83749, M94623, M95200, U09507, U42386, U65592, U94828, U95826, X03505, X15789, X16009, X16490, X53929, X56304, X59520, X60980, X70058, X72307, X78445, X93035, X97227, X99915, Y07519, Z68618; -- Source: Scopus |
