Integrated selective enrichment target - A microtechnology platform for matrix-assisted laser desorption/ionization-mass spectrometry applied on protein biomarkers in prostate diseases Journal Article
| Authors: | Ekström, S.; Wallman, L.; Malm, J.; Becker, C.; Lilja, H.; Laurell, T.; Marko-Varga, G. |
| Article Title: | Integrated selective enrichment target - A microtechnology platform for matrix-assisted laser desorption/ionization-mass spectrometry applied on protein biomarkers in prostate diseases |
| Abstract: | The performance of a miniaturized sample processing platform for matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), manufactured by silicon microfabrication, called integrated selective enrichment target (ISET) technology was evaluated in a biological context. The ISET serves as both sample treatment device and MALDI-MS target, and contains an array of 96 perforated nanovials, which each can be filled with 40 nL of reversed-phase beads. This methodology minimizes the number of sample transfers and the total surface area available for undesired adsorption of the analytes in order to provide high-sensitivity analysis. ISET technology was successfully applied for characterization of proteins coisolated by affinity chromatography of prostate-specific antigen (PSA) from human seminal fluid. The application of ISET sample preparation enabled multiple analyses to be performed on a limited sample volume, which resulted in the discovery that prolactin inducible protein (PIP) was coisolated from the samples. © 2004 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| Keywords: | controlled study; methodology; sensitivity and specificity; sensitivity analysis; proteins; biological marker; prostate specific antigen; protein analysis; biological markers; biotechnology; proteomics; prostate-specific antigen; prostatic neoplasms; microarray analysis; biomarker; carrier proteins; adsorption; nanoparticle; matrix assisted laser desorption ionization time of flight mass spectrometry; glycoproteins; silicon; protein isolation; seminal plasma; miniaturization; prolactin; prostate disease; spectrometry, mass, matrix-assisted laser desorption-ionization; affinity chromatography; phase transition; process technology; electronics; chromatography, affinity; surface property; semen; microspheres; prostatic diseases; chip; analytical equipment; humans; human; male; article; solid-phase extraction; microanalysis |
| Journal Title: | Electrophoresis |
| Volume: | 25 |
| Issue: | 21-22 |
| ISSN: | 0173-0835 |
| Publisher: | Wiley Blackwell |
| Date Published: | 2004-11-01 |
| Start Page: | 3769 |
| End Page: | 3777 |
| Language: | English |
| DOI: | 10.1002/elps.200406094 |
| PROVIDER: | scopus |
| PUBMED: | 15565686 |
| DOI/URL: | |
| Notes: | Electrophoresis -- Cited By (since 1996):32 -- Export Date: 16 June 2014 -- CODEN: ELCTD -- Source: Scopus |
