Real-time cellular analysis for quantitative detection of functional Clostridium difficile toxin in stool Journal Article

  


Authors: Huang, B.; Li, H.; Jin, D.; Stratton, C. W.; Tang, Y. W.
Article Title: Real-time cellular analysis for quantitative detection of functional Clostridium difficile toxin in stool
Abstract: Rapid and accurate diagnosis and monitoring of Clostridium difficile infection (CDI) is critical for patient care and infection control. We will briefly review current laboratory techniques for the diagnosis of CDI and identify aspects needing improvement. We will also introduce a real-time cellular analysis (RTCA) assay developed for the diagnosis and monitoring of CDI using electronic impedance to assess the cell status. The RTCA assay uses impedance measurement to detect minute physiological changes in cells cultured on gold microelectrodes embedded in glass substrates in the bottom of microtiter wells. This assay has been adapted for quantitative detection of C. difficile functional toxin directly from stool specimens. Compared to conventional techniques and molecular assays, the RTCA assay provides a valuable tool for the diagnosis of CDI as well as for the assessment of clinical severity and for monitoring therapeutic efficacies. © 2014 Informa UK, Ltd.
Keywords: laboratory diagnosis; quantitative assay; monitoring; bacterium detection; clostridium difficile infection; detection; bacterium identification; microbiological examination; clostridium difficile; feces; quantification; procedures; toxin; article; real time cellular analysis; real-time cellular analysis; clostridium difficile toxin a; clostridium difficile toxin b
Journal Title: Expert Review of Molecular Diagnostics
Volume: 14
Issue: 3
ISSN: 1473-7159
Publisher: Informa Healthcare  
Date Published: 2014-04-01
Start Page: 281
End Page: 291
Language: English
DOI: 10.1586/14737159.2014.900442
PROVIDER: scopus
PUBMED: 24649817
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84896997306&partnerID=40&md5=3414446eb558f77e1d0478991d64d837
Notes: Export Date: 1 May 2014 -- CODEN: ERMDC -- Source: Scopus
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MSK Authors
  1. Yi-Wei Tang
    188 Tang
  2. Dazhi Jin
    5 Jin
  3. Bin Huang
    7 Huang
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