A phosphate-binding pocket within the platform-PAZ-connector helix cassette of human dicer Journal Article
| Authors: | Tian, Y.; Simanshu, D. K.; Ma, J. B. ; Park, J. E.; Heo, I.; Kim, V. N.; Patel, D. J. |
| Article Title: | A phosphate-binding pocket within the platform-PAZ-connector helix cassette of human dicer |
| Abstract: | We have solved two families of crystal structures of the human Dicer "platform-PAZ-connector helix" cassette in complex with small interfering RNAs (siRNAs). The structures possess two adjacently positioned pockets: a 2 nt 3'-overhang-binding pocket within the PAZ domain (3' pocket) and a phosphate-binding pocket within the platform domain (phosphate pocket). One family of complexes contains a knob-like α-helical protrusion, designated "hDicer-specific helix," that separates the two pockets and orients the bound siRNA away from the surface of Dicer, which could be indicative of a product release/transfer state. In the second complex, the helical protrusion is melted/disordered and the bound siRNA is aligned toward the surface of Dicer, suggestive of a cleavage-competent state. These structures allow us to propose that the transition from the cleavage-competent to the postulated product release/transfer state may involve release of the 5'-phosphate from the phosphate pocket while retaining the 3' overhang in the 3' pocket. © 2014 Elsevier Inc. |
| Journal Title: | Molecular Cell |
| Volume: | 53 |
| Issue: | 4 |
| ISSN: | 1097-2765 |
| Publisher: | Cell Press |
| Date Published: | 2014-02-20 |
| Start Page: | 606 |
| End Page: | 616 |
| Language: | English |
| DOI: | 10.1016/j.molcel.2014.01.003 |
| PROVIDER: | scopus |
| PUBMED: | 24486018 |
| PMCID: | PMC4217634 |
| DOI/URL: | |
| Notes: | Export Date: 2 April 2014 -- CODEN: MOCEF -- Source: Scopus |
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