Influence of free fatty acids on glucose uptake in prostate cancer cells Journal Article
| Authors: | Andersen, K. F.; Divilov, V.; Sevak, K.; Koziorowski, J.; Lewis, J. S.; Pillarsetty, N. |
| Article Title: | Influence of free fatty acids on glucose uptake in prostate cancer cells |
| Abstract: | Introduction: The study focuses on the interaction between glucose and free fatty acids (FFA) in malignant human prostate cancer cell lines by an in vitro observation of uptake of fluoro-2-deoxy- d-glucose (FDG) and acetate. Methods: Human prostate cancer cell lines (PC3, CWR22Rv1, LNCaP, and DU145) were incubated for 2h and 24h in glucose-containing (5.5mM) Dulbecco's Modified Eagle's Medium (DMEM) with varying concentrations of the free fatty acid palmitate (0-1.0mM). Then the cells were incubated with [18F]-FDG (1μCi/mL; 0.037MBq/mL) in DMEM either in presence or absence of glucose and in presence of varying concentrations of palmitate for 1h. Standardized procedures regarding cell counting and measuring for 18F radioactivity were applied. Cell uptake studies with 14C-1-acetate under the same conditions were performed on PC3 cells. Results: In glucose containing media there was significantly increased FDG uptake after 24h incubation in all cell lines, except DU145, when upper physiological levels of palmitate were added. A 4-fold increase of FDG uptake in PC3 cells (15.11% vs. 3.94%/106 cells) was observed in media with 1.0mM palmitate compared to media with no palmitate. The same tendency was observed in PC3 and CWR22Rv1 cells after 2h incubation. In glucose-free media no significant differences in FDG uptake after 24h incubation were observed. The significant differences after 2h incubation all pointed in the direction of increased FDG uptake when palmitate was added. Acetate uptake in PC3 cells was significantly lower when palmitate was added in glucose-free DMEM. No clear tendency when comparing FDG or acetate uptake in the same media at different time points of incubation was observed. Conclusions: Our results indicate a FFA dependent metabolic boost/switch of glucose uptake in PCa, with patterns reflecting the true heterogeneity of the disease. © 2014 Elsevier Inc. |
| Keywords: | metabolism; prostate cancer; glucose; free fatty acid; cell uptake |
| Journal Title: | Nuclear Medicine and Biology |
| Volume: | 41 |
| Issue: | 3 |
| ISSN: | 0969-8051 |
| Publisher: | Elsevier Science Inc. |
| Date Published: | 2014-03-01 |
| Start Page: | 254 |
| End Page: | 258 |
| Language: | English |
| DOI: | 10.1016/j.nucmedbio.2013.12.005 |
| PROVIDER: | scopus |
| PUBMED: | 24440212 |
| PMCID: | PMC4959437 |
| DOI/URL: | |
| Notes: | Export Date: 3 March 2014 -- CODEN: NMBIE -- Source: Scopus |
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133Pillarsetty -
456Lewis -
16Divilov -
11Sevak
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