Stimulation of cell proliferation and expression of matrixmetalloproteinase-1 and interluekin-8 genes in dermal fibroblasts by copper Journal Article
| Authors: | Philips, N.; Hwang, H.; Chauhan, S.; Leonardi, D.; Gonzalez, S. |
| Article Title: | Stimulation of cell proliferation and expression of matrixmetalloproteinase-1 and interluekin-8 genes in dermal fibroblasts by copper |
| Abstract: | Copper is essential to wound healing as well as a widespread environmental pollutant, with skin aging potential. Wound healing and skin aging are facilitated by matrixmetalloproteinases (MMP), which remodel the extracellular matrix, and interleukin-8 (IL-8), linked with copper. This research investigated the mechanism to copper's role in wound healing or skin aging by regulation of MMP-1 and IL-8 genes. It examined the dose-responsive effects of copper on MMP-1, -2, and -9 activities; MMP-1 and IL-8 gene regulation at protein, mRNA, and promoter levels; tissue inhibitor of matrixmetalloproteinases-1 (TIMP-1) expression; and cell proliferation. Copper stimulated cell proliferation and the expression of MMP-1 and IL-8 genes at the protein, mRNA, and promoter levels, indicating transcriptional regulation, without significantly altering TIMP-1. The research suggests that copper facilitates wound healing as well as skin aging via the induction of MMP-1 expression, with limiting MMP effect at the higher concentrations through enhanced IL-8 expression, which favors extracellular matrix deposition. © 2010 Informa UK Ltd. |
| Keywords: | controlled study; protein expression; human cell; promoter region; genetics; mutation; dose response; cell proliferation; metabolism; cell survival; cells, cultured; gene expression; gelatinase b; interleukin 8; drug effect; skin; wound healing; gene expression regulation; enzyme regulation; transcription regulation; interleukin-8; cell culture; infant, newborn; messenger rna; rna, messenger; newborn; matrix metalloproteinase; fibroblast; fibroblasts; gene control; aging; interstitial collagenase; copper; matrix metalloproteinase 1; tissue inhibitor of metalloproteinase 1; gelatinase a; skin fibroblast; skin aging; matrix metalloproteinase inhibitor; matrixmetalloprotienase; cutaneous parameters; pollutant; environmental pollutants; tissue inhibitor of metalloproteinase-1 |
| Journal Title: | Connective Tissue Research |
| Volume: | 51 |
| Issue: | 3 |
| ISSN: | 0300-8207 |
| Publisher: | Informa Healthcare |
| Date Published: | 2010-05-19 |
| Start Page: | 224 |
| End Page: | 229 |
| Language: | English |
| DOI: | 10.3109/03008200903288431 |
| PUBMED: | 20053132 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Export Date: 20 April 2011" - "CODEN: CVTRB" - "Source: Scopus" |
