Distinct pattern of matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 mRNA expression in human colorectal cancer and liver metastases Journal Article
| Authors: | Zeng, Z. S.; Guillem, J. G. |
| Article Title: | Distinct pattern of matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 mRNA expression in human colorectal cancer and liver metastases |
| Abstract: | The matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) are perceived as essential for tumour invasion and metastasis. In the present study, we compare the topographical pattern of MMP-9 and TIMP-1 expression in colorectal cancer and liver metastasis by in situ hybridisation. TIMP-1 mRNA was detected in all 26 colorectal cancers examined, while only 18 out of 26 (69.2%) were positive for MMP-9. Both MMP-9 and TIMP-1 mRNA were observed in all ten liver metastases but were absent in three adenomas and in all normal colonic mucosa and liver. There was no association between MMP-9 or TIMP-1 mRNA expression and degree of differentiation or size of Tumours. MMP-9 and TIMP-1 mRNA were similarly observed in the peritumour stroma cells rather than in tumour cells themselves. MMP-9 mRNA positive cells were round and identified as macrophages by immunostaining with an anti-macrophage antibody (KP1), while TIMP-1, mRNA was detected in spindle-shaped stromal cells. In liver metastases, MMP-9 localised within peritumour stroma or at the interface between the tumour stroma and normal liver, whereas TIMP-1 mRNA was located throughout the malignant tumour stroma. Our data demonstrate a distinct pattern of MMP-9 and TIMP-1 mRNA expression in colorectal cancer and liver metastases suggesting distinct cellular origins as well as separate patterns of regulation. © 1995 Stockton Press. All rights reserved. |
| Keywords: | immunohistochemistry; adult; human tissue; aged; aged, 80 and over; liver neoplasms; comparative study; colorectal cancer; gene expression; gelatinase b; tumor differentiation; carcinogenesis; colorectal neoplasms; in situ hybridization; liver metastasis; messenger rna; rna, messenger; matrix metalloproteinase; tumor cell; macrophage; stroma cell; blotting, northern; collagenases; glycoproteins; tissue inhibitor of metalloproteinase; tissue inhibitor of metalloproteinases; northern blotting; middle age; colon mucosa; topography; human; male; female; priority journal; article; support, non-u.s. gov't; in situ hvbridisation; type iv collagenase |
| Journal Title: | British Journal of Cancer |
| Volume: | 72 |
| Issue: | 3 |
| ISSN: | 0007-0920 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1995-09-01 |
| Start Page: | 575 |
| End Page: | 582 |
| Language: | English |
| DOI: | 10.1038/bjc.1995.376 |
| PUBMED: | 7669564 |
| PROVIDER: | scopus |
| PMCID: | PMC2033901 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
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