A primer on clinical applications and assays using urine: Focus on analysis of plasma cell dyscrasias using automated electrophoresis and immunofixation Journal Article
| Authors: | Olivieri, B.; Rai, A. J. |
| Article Title: | A primer on clinical applications and assays using urine: Focus on analysis of plasma cell dyscrasias using automated electrophoresis and immunofixation |
| Abstract: | Urine is a noninvasive sample that is ideal for screening, because it is easy to collect, cost-effective, and can provide a wealth of information on a patient's health status. We provide a brief discussion on the anatomy and physiology of the kidney, a concise overview on B and T cells as key mediators of the immune system, and then delve into the various B-cell neoplasms. This discussion details Waldenstrom's macroglobulinemia, plasmacytoma, heavy chain disease, amyloidosis, and multiple myeloma. Of primary clinical importance from a technical perspective, two commonly applied techniques for the separation and characterization of urine proteins include urine protein electrophoresis and urine protein immunofixation. Procedural details for both techniques are provided herein. |
| Keywords: | methodology; automation; immunology; staining; urine; immunoassay; urinalysis; electrophoresis; staining and labeling; paraproteinemia; paraproteinemias |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 641 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2010-01-01 |
| Start Page: | 13 |
| End Page: | 26 |
| Language: | English |
| DOI: | 10.1007/978-1-60761-711-2_2 |
| PUBMED: | 20407939 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Chapter 2 in "The Urinary Proteome: Methods and Protocols" (ISBN: 978-1-60761-710-5) --- Export Date: 20 April 2011 - Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work