A prospective, multi-Institutional diagnostic trial to determine pathologist accuracy in estimation of percentage of malignant cells Journal Article


Authors: Viray, H.; Li, K.; Long, T. A.; Vasalos, P.; Bridge, J. A.; Jennings, L. J.; Halling, K. C.; Hameed, M.; Rimm, D. L.
Article Title: A prospective, multi-Institutional diagnostic trial to determine pathologist accuracy in estimation of percentage of malignant cells
Abstract: Context.-The fraction of malignant cells in tumor tissue submitted for tests of genetic alterations is a critical variable in testing accuracy. That fraction is currently determined by pathologist visual estimation of the percentage of malignant cells. Inaccuracy could lead to a false-negative test result. Objective.-To describe a prospective, multi-institutional study to determine pathologist estimation accuracy. Design.-Ten320 magnification images of hematoxylineosin-stained colon tissue specimens were sent as an educational component of the College of American Pathologists KRAS-B 2011 Survey. Data from 194 labs were analyzed and compared to a criterion standard with comprehensive manual nuclear counts. Results.-Survey responses indicated low interlaboratory precision of pathologist estimation, but mean estimates were fairly accurate. A total of 5 of the 10 cases assessed showed more than 10% of respondents overestimating in a manner that could lead to false-negative test results. Conclusions.-The significance of estimation errors resulting in molecular testing failures with implications for patient care is unknown, but the current study suggests false-negative test results may occur.
Journal Title: Archives of Pathology & Laboratory Medicine
Volume: 137
Issue: 11
ISSN: 0003-9985
Publisher: College of American Pathologists  
Date Published: 2013-11-01
Start Page: 1545
End Page: 1549
Language: English
DOI: 10.5858/arpa.2012-0561-CP
PROVIDER: scopus
PUBMED: 24168492
DOI/URL:
Notes: --- - "Export Date: 2 December 2013" - "CODEN: ARPAA" - "Source: Scopus"
Altmetric
Citation Impact
MSK Authors
  1. Meera Hameed
    214 Hameed