Quantitative imaging of disease signatures through radioactive decay signal conversion Journal Article
| Authors: | Thorek, D. L. J.; Ogirala, A.; Beattie, B. J.; Grimm, J. |
| Article Title: | Quantitative imaging of disease signatures through radioactive decay signal conversion |
| Abstract: | In the era of personalized medicine, there is an urgent need for in vivo techniques able to sensitively detect and quantify molecular activities. Sensitive imaging of gamma rays is widely used; however, radioactive decay is a physical constant, and its signal is independent of biological interactions. Here, we introduce a framework of previously uncharacterized targeted and activatable probes that are excited by a nuclear decay-derived signal to identify and measure molecular signatures of disease. We accomplished this by using Cerenkov luminescence, the light produced by β-particle-emitting radionuclides such as clinical positron emission tomography (PET) tracers. Disease markers were detected using nanoparticles to produce secondary Cerenkov-induced fluorescence. This approach reduces background signal compared to conventional fluorescence imaging. In addition to tumor identification from a conventional PET scan, we demonstrate the medical utility of our approach by quantitatively determining prognostically relevant enzymatic activity. This technique can be applied to monitor other markers and represents a shift toward activatable nuclear medicine agents. © 2013 Nature America, Inc. All rights reserved. |
| Journal Title: | Nature Medicine |
| Volume: | 19 |
| Issue: | 10 |
| ISSN: | 1078-8956 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2013-10-01 |
| Start Page: | 1345 |
| End Page: | 1350 |
| Language: | English |
| DOI: | 10.1038/nm.3323 |
| PROVIDER: | scopus |
| PMCID: | PMC3795968 |
| PUBMED: | 24013701 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 2 December 2013" - "CODEN: NAMEF" - "Source: Scopus" |
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