Quantitative imaging of disease signatures through radioactive decay signal conversion Journal Article

Authors: Thorek, D. L. J.; Ogirala, A.; Beattie, B. J.; Grimm, J.
Article Title: Quantitative imaging of disease signatures through radioactive decay signal conversion
Abstract: In the era of personalized medicine, there is an urgent need for in vivo techniques able to sensitively detect and quantify molecular activities. Sensitive imaging of gamma rays is widely used; however, radioactive decay is a physical constant, and its signal is independent of biological interactions. Here, we introduce a framework of previously uncharacterized targeted and activatable probes that are excited by a nuclear decay-derived signal to identify and measure molecular signatures of disease. We accomplished this by using Cerenkov luminescence, the light produced by β-particle-emitting radionuclides such as clinical positron emission tomography (PET) tracers. Disease markers were detected using nanoparticles to produce secondary Cerenkov-induced fluorescence. This approach reduces background signal compared to conventional fluorescence imaging. In addition to tumor identification from a conventional PET scan, we demonstrate the medical utility of our approach by quantitatively determining prognostically relevant enzymatic activity. This technique can be applied to monitor other markers and represents a shift toward activatable nuclear medicine agents. © 2013 Nature America, Inc. All rights reserved.
Journal Title: Nature Medicine
Volume: 19
Issue: 10
ISSN: 1078-8956
Publisher: Nature Publishing Group  
Date Published: 2013-10-01
Start Page: 1345
End Page: 1350
Language: English
DOI: 10.1038/nm.3323
PROVIDER: scopus
PMCID: PMC3795968
PUBMED: 24013701
Notes: --- - "Export Date: 2 December 2013" - "CODEN: NAMEF" - "Source: Scopus"
Citation Impact
MSK Authors
  1. Daniel Thorek
    20 Thorek
  2. Jan Grimm
    62 Grimm
  3. Bradley Beattie
    117 Beattie
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