Concurrent visualization of trafficking, expansion, and activation of T lymphocytes and T-cell precursors in vivo Journal Article

Authors: Na, I. K.; Markley, J. C.; Tsai, J. J.; Yim, N. L.; Beattie, B. J.; Klose, A. D.; Holland, A. M.; Ghosh, A.; Rao, U. K.; Stephan, M. T.; Serganova, I.; Santos, E. B.; Brentjens, R. J.; Blasberg, R. G.; Sadelain, M.; van den Brink, M. R. M.
Article Title: Concurrent visualization of trafficking, expansion, and activation of T lymphocytes and T-cell precursors in vivo
Abstract: We have developed a dual bioluminescent reporter system allowing noninvasive, concomitant imaging of T-cell trafficking, expansion, and activation of nuclear factor of activated T cells (NFAT) in vivo.NFAT activation plays an important role in T-cell activation and T-cell development. Therefore we used this system to determine spatial-temporal activation patterns of (1) proliferating T lymphocytes during graft-versus-host disease (GVHD) and (2) T-cell precursors during T-cell development after allogeneic hematopoietic stem cell transplantation (HSCT). In the first days after HSCT, donor T cells migrated to the peripheral lymph nodes and the intestines, whereas the NFAT activation was dominant in the intestines, suggesting an important role for the intestines in the early stages of alloactivation during development of GVHD. After adoptive transfer of in vitro-derived T-cell receptor (TCR) H-Y transgenic T-cell precursors into B6 (H-2b) hosts of both sexes, NFAT signaling and development into CD4+ or CD8+ single-positive cells could only be detected in the thymus of female recipients indicating either absence of positive selection or prompt depletion of double-positive thymocytes in the male recipients. Because NFAT plays an important role in a wide range of cell types, our system could provide new insights into a variety of biologic processes. © 2010 by The American Society of Hematology.
Keywords: controlled study; nonhuman; cd8 antigen; t lymphocyte; animal cell; mouse; animal experiment; animal model; hematopoietic stem cell transplantation; t lymphocyte receptor; pre t lymphocyte; thymus; lymph node; graft versus host reaction; cell migration; cd4 antigen; thymocyte; intestine; t lymphocyte activation; transcription factor nfat
Journal Title: Blood
Volume: 116
Issue: 11
ISSN: 0006-4971
Publisher: American Society of Hematology  
Date Published: 2010-09-16
Start Page: e18
End Page: e25
Language: English
DOI: 10.1182/blood-2009-12-259432
PROVIDER: scopus
PMCID: PMC3173994
PUBMED: 20511541
Notes: --- - "Cited By (since 1996): 1" - "Export Date: 20 April 2011" - "CODEN: BLOOA" - "Source: Scopus"
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MSK Authors
  1. Ronald G Blasberg
    242 Blasberg
  2. Renier J Brentjens
    189 Brentjens
  3. Il-Kang Na
    24 Na
  4. Elmer B Santos
    25 Santos
  5. Michel W J Sadelain
    453 Sadelain
  6. Jennifer Tsai
    44 Tsai
  7. Arnab Ghosh
    39 Ghosh
  8. Bradley Beattie
    106 Beattie
  9. Uttam Keshav Rao
    28 Rao
  10. Nury Yim
    25 Yim
  11. Amanda M Holland
    52 Holland