Vertebrate cells genetically deficient for Cdc14A or Cdc14B retain DNA damage checkpoint proficiency but are impaired in DNA repair Journal Article

   


Authors: Mocciaro, A.; Berdougo, E.; Zeng, K.; Black, E.; Vagnarelli, P.; Earnshaw, W.; Gillespie, D.; Jallepalli, P.; Schiebel, E.
Article Title: Vertebrate cells genetically deficient for Cdc14A or Cdc14B retain DNA damage checkpoint proficiency but are impaired in DNA repair
Abstract: A recent study suggested that human Cdc14B phosphatase has a central function in the G2 DNA damage checkpoint. In this study, we show that chicken DT40, human HCT116, and human telomerase reverse transcription-immortalized retinal pigment epithelial cells deleted for the Cdc14A or Cdc14B gene are DNA damage checkpoint proficient and arrest efficiently in G2 in response to irradiation. Cdc14A knockout (KO) or Cdc14B-KO cells also maintain normal levels of Chk1 and Chk2 activation after irradiation. Surprisingly, however, irradiation-induced γ-H2A.X foci and DNA double-strand breaks persist longer in Cdc14A-KO or Cdc14B-KO cells than controls, suggesting that Cdc14 phosphatases are required for efficient DNA repair. © 2010 Mocciaro et al.
Keywords: controlled study; unclassified drug; nonhuman; flow cytometry; animal cell; animals; cell cycle proteins; dna damage; cells, cultured; dna repair; protein kinases; hct116 cells; gene locus; enzyme activation; cell line, tumor; dna strand breakage; vertebrata; double stranded dna; protein-serine-threonine kinases; nucleotide sequence; irradiation; telomerase reverse transcriptase; checkpoint kinase 2; cell cycle g2 phase; dual-specificity phosphatases; checkpoint kinase 1; histone h2a; phosphoric monoester hydrolases; chickens; phosphoprotein phosphatase cdc14; phosphoprotein phosphatase cdc14a; phosphoprotein phosphatase cdc14b; pigment epithelium
Journal Title: Journal of Cell Biology
Volume: 189
Issue: 4
ISSN: 0021-9525
Publisher: Rockefeller University Press  
Date Published: 2010-05-17
Start Page: 631
End Page: 639
Language: English
DOI: 10.1083/jcb.200910057
PUBMED: 20479464
PROVIDER: scopus
PMCID: PMC2872905
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-77952376111&partnerID=40&md5=0525f2891b6ff3f3c0012188bbfe1900
Notes: --- - "Cited By (since 1996): 7" - "Export Date: 20 April 2011" - "CODEN: JCLBA" - "Source: Scopus"
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MSK Authors
  1. Prasad V Jallepalli
    34 Jallepalli
  2. Eli Berdougo
    4 Berdougo
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