Fibronectin and integrin alpha 5 play essential roles in the development of the cardiac neural crest Journal Article
| Authors: | Mittal, A.; Pulina, M.; Hou, S. Y.; Astrof, S. |
| Article Title: | Fibronectin and integrin alpha 5 play essential roles in the development of the cardiac neural crest |
| Abstract: | Cardiac neural crest (CNC) plays a requisite role during cardiovascular development and defects in the formation of CNC-derived structures underlie several common forms of human congenital birth defects. Migration of the CNC cells to their destinations as well as expansion and maintenance of these cells are important for the normal development of the cardiac outflow tract and aortic arch arteries; however, molecular mechanisms regulating these processes are not well-understood. Fibronectin (FN) protein is present along neural crest migration paths and neural crest cells migrate when plated on FN in vitro; therefore, we tested the role of FN during the development of the CNC in vivo. Our analysis of the fate of the neural crest shows that CNC cells reach their destinations in the branchial arches and the cardiac outflow tract in the absence of FN or its cellular receptor integrin α5β1. However, we found that FN and integrin α5 modulate CNC proliferation and survival, and are required for the presence of normal numbers of CNC cells at their destinations. © 2010 Elsevier Ireland Ltd. |
| Keywords: | survival; controlled study; nonhuman; cell proliferation; animal cell; mouse; animal tissue; cell survival; embryo; cell fate; embryo development; in vivo study; cell migration; cellular distribution; cell count; neural crest; proliferation; cell expansion; heart development; fibronectin; cardiac neural crest; integrin alpha5; alpha5 integrin; very late activation antigen 5; branchial arch; heart left ventricle outflow tract; heart right ventricle outflow tract |
| Journal Title: | Mechanisms of Development |
| Volume: | 127 |
| Issue: | 9 |
| ISSN: | 0925-4773 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2010-09-01 |
| Start Page: | 472 |
| End Page: | 484 |
| Language: | English |
| DOI: | 10.1016/j.mod.2010.08.005 |
| PROVIDER: | scopus |
| PUBMED: | 20807571 |
| PMCID: | PMC3233353 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 20 April 2011" - "CODEN: MEDVE" - "Source: Scopus" |
