p21cip/WAF is a key regulator of long-term radiation damage in mesenchyme-derived tissues Journal Article
| Authors: | Mehrara, B. J.; Avraham, T.; Soares, M.; Fernandez, J. G.; Yan, A.; Zampell, J. C.; Andrade, V. P.; Cordeiro, A. P.; Sorrento, C. M. |
| Article Title: | p21cip/WAF is a key regulator of long-term radiation damage in mesenchyme-derived tissues |
| Abstract: | This study aimed to determine the mechanisms responsible for long-term tissue damage following radiation injury. We irradiated p21-knockout (p21 -/-) and wild-type (WT) mice and determined the long-term deleterious effects of this intervention on mesenchymederived tissues. In addition, we explored the mechanisms of radiation-induced mesenchymal stem cell (MSC) dysfunction in isolated bone marrow-derived cells. p21 expression was chronically elevated >200-fold in irradiated tissues. Loss of p21 function resulted in a >4-fold increase in the number of skin MSCs remaining after radiation. p21-/- mice had significantly less radiation damage, including 6-fold less scarring, 40% increased growth potential, and 4-fold more hypertrophic chondrocytes in the epiphyseal plate (P<0.01). Irradiated p21-/- MSCs had 4-fold increased potential for bone or fat differentiation, 4-fold greater proliferation rate, and nearly 7-fold lower senescence as compared to WT MSCs (P<0.01). Ectopic expression of p21 in knockout cells decreased proliferation and differentiation potential and recapitulated the WT phenotype. Loss of p21 function markedly decreases the deleterious effects of radiation injury in mesenchyme-derived tissues and preserves tissue-derived MSCs. In addition, p21 is a critical regulator of MSC proliferation, differentiation, and senescence both at baseline and in response to radiation. © FASEB. |
| Keywords: | controlled study; protein expression; genetics; nonhuman; protein function; cell proliferation; animal cell; mouse; phenotype; animal; cytology; metabolism; mouse mutant; animals; mice; mice, knockout; cells, cultured; mus; cell cycle; reverse transcription polymerase chain reaction; apoptosis; cell growth; animal experiment; cell differentiation; radiation injury; wild type; radiation exposure; blotting, western; whole body radiation; cell culture; reverse transcriptase polymerase chain reaction; cell damage; western blotting; bone; scar formation; ionizing radiation; radiation, ionizing; cell cycle arrest; mesenchymal stem cell; cell count; bone marrow cell; gene silencing; cdkn1a protein, mouse; cyclin dependent kinase inhibitor 1a; cyclin-dependent kinase inhibitor p21; protein p21; tissue injury; cell aging; mesenchyme; p21; differentiation; fat; mesenchymal stem cells; skin fibrosis; cartilage cell; epiphysis plate |
| Journal Title: | FASEB Journal |
| Volume: | 24 |
| Issue: | 12 |
| ISSN: | 0892-6638 |
| Publisher: | Federation of American Societies for Experimental Biology |
| Date Published: | 2010-12-01 |
| Start Page: | 4877 |
| End Page: | 4888 |
| Language: | English |
| DOI: | 10.1096/fj.10-155762 |
| PUBMED: | 20720160 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Export Date: 20 April 2011" - "CODEN: FAJOE" - "Source: Scopus" |
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