Fractionated doses of ionizing radiation confer protection to mesenchymal stem cell pluripotency Journal Article
| Authors: | Clavin, N. W.; Fernandez, J.; Schonmeyr, B. H.; Soares, M. A.; Mehrara, B. J. |
| Article Title: | Fractionated doses of ionizing radiation confer protection to mesenchymal stem cell pluripotency |
| Abstract: | BACKGROUND: Although it is clear that radiation therapy can cause tissue injury, the degree of injury that is observed clinically can be highly variable. It is possible that variability in the methods by which ionizing radiation is delivered can contribute to some of the observed variability. Thus, the purpose of this study was to assess the effects of various fractionation schedules on the growth and differentiation potential of isolated mesenchymal stem cells in vitro. METHODS: Isolated mesenchymal stem cells (triplicate studies) were exposed to a dose of 12 Gy of ionizing radiation as a single dose, in two doses of 6 Gy, or in six doses of 2 Gy. Cellular proliferation and the potential for differentiation along the bone and fat lineage were assessed. Potential mechanisms for injury and protection were evaluated by analyzing the expression of p21 and manganese superoxide dismutase. RESULTS: Delivery of radiation in multiple doses confers significant radioprotection to mesenchymal stem cell proliferation and potential for differentiation. In contrast, delivery of 12 Gy of radiation as a single dose or as two equal doses of 6 Gy results in marked deficiencies in cellular proliferation and potential for multilineage cellular differentiation. CONCLUSIONS: The authors have demonstrated that even minor alterations in fractionation of radiation dose can result in significant effects on the potential of mesenchymal stem cells to differentiate. These findings imply that at least some of the variability in tissue damage after radiation therapy observed clinically may be attributable to differences in the delivery of ionizing radiation. Copyright © 2008 by the American Society of Plastic Surgeons. |
| Keywords: | controlled study; protein expression; nonhuman; radiation dose; cell proliferation; animal cell; mouse; animal; cytology; animals; mice; cell division; reverse transcription polymerase chain reaction; apoptosis; cell growth; cell differentiation; in vitro study; radiation injury; mice, inbred balb c; radiation exposure; physiology; radiation dosage; cell lineage; rna; bagg albino mouse; reverse transcriptase polymerase chain reaction; radiation dose fractionation; bone; cell isolation; ionizing radiation; mesenchymal stem cell; pluripotent stem cell; pluripotent stem cells; bone marrow cell; bone development; bone and bones; osteogenesis; protein p21; cell protection; tissue injury; lipogenesis; radiation protection; mesenchymal stem cells; manganese superoxide dismutase; lipid storage; lipoprotein lipase |
| Journal Title: | Plastic and Reconstructive Surgery |
| Volume: | 122 |
| Issue: | 3 |
| ISSN: | 0032-1052 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 2008-09-01 |
| Start Page: | 739 |
| End Page: | 748 |
| Language: | English |
| DOI: | 10.1097/PRS.0b013e318180edaa |
| PUBMED: | 18766036 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 8" - "Export Date: 17 November 2011" - "CODEN: PRSUA" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work