Live imaging, identifying, and tracking single cells in complex populations in vivo and ex vivo Journal Article

   


Authors: Kang, M.; Xenopoulos, P.; Munoz Descalzo, S.; Lou, X.; Hadjantonakis, A. K.
Article Title: Live imaging, identifying, and tracking single cells in complex populations in vivo and ex vivo
Abstract: Advances in optical imaging technologies combined with the use of genetically encoded fluorescent proteins have enabled the visualization of stem cells over extensive periods of time in vivo and ex vivo. The generation of genetically encoded fluorescent protein reporters that are fused with subcellularly localized proteins, such as human histone H2B, has made it possible to direct fluorescent protein reporters to specific subcellular structures and identify single cells in complex populations. This facilitates the visualization of cellular behaviors such as division, movement, and apoptosis at a single-cell resolution and, in principle, allows the prospective and retrospective tracking towards determining the lineage of each cell. © Springer Science+Business Media New York 2013.
Keywords: unclassified drug; nonhuman; animal cell; mouse; cell division; apoptosis; image analysis; embryo; embryonic stem cell; cell protein; protein binding; cell motion; in vivo study; cell population; genetic engineering; reporter gene; embryonic stem cells; ex vivo study; computer program; histone h2b; fluorescence imaging; cell tracking; live imaging; mouse embryo; cell labeling; fluorescent protein; ex utero culture; peptides and proteins; time lapse; automated image analysis; in vitro culture
Journal Title: Methods in Molecular Biology
Volume: 1052
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2013-01-01
Start Page: 109
End Page: 123
Language: English
DOI: 10.1007/7651_2013_19
PROVIDER: scopus
PUBMED: 23640250
PMCID: PMC3967584
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84883227073&partnerID=40&md5=4cd39c6f5cf35cb81bdfdeec20a83e75
Notes: Chapter 10 in "Imaging and Tracking Stem Cells: Methods and Protocols" (ISBN: 978-1-62703-558-3)--- Export Date: 1 October 2013 - Source: Scopus
Altmetric
What is Altmetric?
Citation Impact
What is Dimensions Citation Badge?
BMJ Impact Analytics
MSK Authors
  1. Anna-Katerina Hadjantonakis
    191 Hadjantonakis
  2. Panagiotis Xenopoulos
    11 Xenopoulos
  3. Silvia Munoz Descalzo
    4 Munoz Descalzo
  4. Xinghua Lou
    7 Lou
  5. Min Jung Kang
    12 Kang
Related MSK Work