Oncogenic H-Ras and PI3K signaling can inhibit e-cadherin-dependent apoptosis and promote cell survival after photodynamic therapy in mouse keratinocytes Journal Article
| Authors: | Espada, J.; Galaz, S.; Sanz-Rodriguez, F.; Blázquez-Castro, A.; Stockert, J. C.; Bagazgoitia, L.; Jaén, P.; Gonzalez, S.; Cano, A.; Juarranz, A. |
| Article Title: | Oncogenic H-Ras and PI3K signaling can inhibit e-cadherin-dependent apoptosis and promote cell survival after photodynamic therapy in mouse keratinocytes |
| Abstract: | Maintenance of E-cadherin mediated cell-cell contacts is often required for the survival of epithelial cells and tissues. Here we report that oncogenic activation of H-Ras in murine keratinocytes can prevent cell death induced by immunological disruption of E-cadherin adhesion. A similar situation was observed in cells showing constitutive activation of the p110a catalytic subunit of class IA PI3K. This protective effect is associated with b-catenin-dependent transcription and with activation of survival factor Akt/PKB. In addition, we induced cell death by employing photodynamic therapy, using Zn-phthalocyanine as a photosensitizer that targets E-cadherin adhesion complexes. We have found that cell death based on this photodynamic action is also bypassed in cells showing constitutive activation of H-Ras and p110α. Taken together, these results indicate that H-Ras/PI3K/Akt signaling plays a key role in cell survival mediated by E-cadherin cell-cell contacts. © 2008 Wiley-Liss, Inc. |
| Keywords: | signal transduction; protein kinase b; controlled study; genetics; nonhuman; animal cell; mouse; animal; cytology; metabolism; animals; mice; cell death; cell survival; cell cycle; apoptosis; cell line; genetic transcription; enzyme activation; phosphatidylinositol 3 kinase; uvomorulin; oncogene h ras; mice, inbred balb c; keratinocyte; radiation exposure; physiology; bagg albino mouse; gene disruption; rat; 1-phosphatidylinositol 3-kinase; proto-oncogene proteins c-akt; murinae; double stranded dna break; upregulation; cell junction; indoles; beta catenin; phthalocyanine zinc; procaspase 3; protein p110; cadherin; indole derivative; phthalocyanine; protein p21; radiosensitizing agent; alpha chain; cell activation; cell adhesion; cell communication; cell protection; enzyme release; photodynamic therapy; photodynamics; photochemotherapy; cadherins; intercellular junctions; keratinocytes; proto-oncogene proteins p21(ras); radiation-sensitizing agents |
| Journal Title: | Journal of Cellular Physiology |
| Volume: | 219 |
| Issue: | 1 |
| ISSN: | 0021-9541 |
| Publisher: | John Wiley & Sons, Inc. |
| Date Published: | 2009-04-01 |
| Start Page: | 84 |
| End Page: | 93 |
| Language: | English |
| DOI: | 10.1002/jcp.21652 |
| PUBMED: | 19065634 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 30 November 2010" - "CODEN: JCLLA" - "Source: Scopus" |
