Vinculin is associated with the E-cadherin adhesion complex Journal Article
| Authors: | Hazan, R. B.; Kang, L.; Roe, S.; Borgen, P. I.; Rimm, D. L. |
| Article Title: | Vinculin is associated with the E-cadherin adhesion complex |
| Abstract: | Cadherins mediate calcium-dependent cell-cell adhesion, and this activity is regulated by cytoplasmic interactions between cadherins, catenins, and the actin-based cytoskeleton. α-Catenin plays a critical role in the transmembrane anchorage of cadherins, and deletion of α-catenin has been shown to inactivate cadherin-mediated adhesion, resulting in a nonadhesive phenotype. Here we show that serum starvation increases E- cadherin expression and induces E-cadherin-dependent adhesion in the MDA-MB- 468 breast cancer cell line. This adhesion occurred despite a lack of α- catenin expression, which was caused by mutations in the α-catenin gone. Coprecipitation analysis suggests that this adhesion may be mediated by cytoplasmic connections from cadherins to the cytoskeleton involving vinculin. A high level of vinculin associated with E-cadherin immunoprecipitates was observed in MDA-MB-468 cells. In contrast, vinculin was not detected in E-cadherin complexes in the A431 and MCF-7 epithelial carcinoma cell lines, which express α-catenin. However, in reciprocal immunoprecipitations using anti-vinculin antibodies, E-cadherin associated strongly with vinculin in MDA-MB-468 cells and, to a lesser extent, in A431 and MCF-7 cells. These results suggest that both α-catenin and vinculin may be present in the adhesion complex. To test the hypothesis that vinculin associates with E-cadherin complexes via β-catenin, excess recombinant β- catenin or α-catenin fusion protein was added to MDA-MB-468 cell lysates. Both specifically inhibited the coprecipitation of E-cadherin with vinculin, suggesting competition for the same binding site. These results suggest that vinculin plays a role in the establishment or regulation of the cadherin- based cell adhesion complex by direct interaction with β-catenin. |
| Keywords: | protein expression; human cell; breast cancer; protein binding; cancer cell culture; tumor cells, cultured; uvomorulin; amino acid sequence; molecular sequence data; hybrid protein; recombinant protein; immunoprecipitation; cell interaction; cell adhesion; cadherins; cytoskeleton; cytoskeletal proteins; competitive inhibition; alpha catenin; culture media, serum-free; vinculin; humans; human; priority journal; article |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 272 |
| Issue: | 51 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1997-12-19 |
| Start Page: | 32448 |
| End Page: | 32453 |
| Language: | English |
| DOI: | 10.1074/jbc.272.51.32448 |
| PUBMED: | 9405455 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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