High 18F-FDG uptake in microscopic peritoneal tumors requires physiologic hypoxia Journal Article
| Authors: | Li, X. F. ; Ma, Y.; Sun, X.; Humm, J. L.; Ling, C. C.; O'Donoghue, J. A. |
| Article Title: | High 18F-FDG uptake in microscopic peritoneal tumors requires physiologic hypoxia |
| Abstract: | The objective of this study was to examine 18F-FDG uptake in microscopic tumors grown intraperitoneally in nude mice and to relate this to physiologic hypoxia and glucose transporter-1 (GLUT-1) expression. Methods: Human colon cancer HT29 and HCT-8 cells were injected intraperitoneally into nude mice to generate disseminated tumors of varying sizes. After overnight fasting, animals, breathing either air or carbogen (95% O2 + 5% CO2), were intravenously administered 18F-FDG together with the hypoxia marker pimonidazole and cellular proliferation marker bromodeoxyuridine 1 h before sacrifice. Hoechst 33342, a perfusion marker, was administered 1 min before sacrifice. After sacrifice, the intratumoral distribution of 18F-FDG was assessed by digital autoradiography of frozen tissue sections. Intratumoral distribution was compared with the distributions of pimonidazole, GLUT-1 expression, bromodeoxyuridine, and Hoechst 33342 as visualized by immunofluorescent microscopy. Results: Small tumors (diameter, <1 mm) had high 18F-FDG accumulation and were severely hypoxic, with high GLUT-1 expression. Larger tumors (diameter, 1-4 mm) generally had low 18F-FDG accumulation and were not significantly hypoxic, with low GLUT-1 expression. Carbogen breathing significantly decreased 18F-FDG accumulation and tumor hypoxia in microscopic tumors but had little effect on GLUT-1 expression. Conclusion: There was high 18F-FDG uptake in microscopic tumors that was spatially associated with physiologic hypoxia and high GLUT-1 expression. This enhanced uptake was abrogated by carbogen breathing, indicating that in the absence of physiologic hypoxia, high GLUT-1 expression, by itself, was insufficient to ensure high 18F-FDG uptake. Copyright © 2010 by the Society of Nuclear Medicine, Inc. |
| Keywords: | controlled study; protein expression; human cell; genetics; disseminated cancer; nonhuman; pathophysiology; cell proliferation; mouse; animal; metabolism; animals; mice; animal tissue; peritoneal neoplasms; tumor volume; oxygen; animal experiment; animal model; drug effect; pathology; hypoxia; gene expression regulation; fluorescent antibody technique; gene expression regulation, neoplastic; cancer cell; fluorodeoxyglucose f 18; fluorodeoxyglucose f18; micrometastasis; pimonidazole; respiration; ascites tumor; cell strain ht29; ht29 cells; anoxia; biological transport; peritoneum tumor; nitroimidazole derivative; autoradiography; nitroimidazoles; glucose transporter 1; glucose transporter type 1; carbogen; carbon dioxide; digital imaging; glucose metabolism; transport at the cellular level; 18f- fluorodeoxyglucose; breathing; air |
| Journal Title: | Journal of Nuclear Medicine |
| Volume: | 51 |
| Issue: | 4 |
| ISSN: | 0161-5505 |
| Publisher: | Society of Nuclear Medicine |
| Date Published: | 2010-04-01 |
| Start Page: | 632 |
| End Page: | 638 |
| Language: | English |
| DOI: | 10.2967/jnumed.109.071233 |
| PUBMED: | 20351353 |
| PROVIDER: | scopus |
| PMCID: | PMC2917184 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 20 April 2011" - "CODEN: JNMEA" - "Source: Scopus" |
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