Dependence of FDG uptake on tumor microenvironment Journal Article
| Authors: | Pugachev, A.; Ruan, S.; Carlin, S.; Larson, S. M.; Campa, J.; Ling, C. C.; Humm, J. L. |
| Article Title: | Dependence of FDG uptake on tumor microenvironment |
| Abstract: | Purpose: To investigate the factors affecting the 18F- fluorodeoxyglucose (18F-FDG) uptake in tumors at a microscopic level, by correlating it with tumor hypoxia, cellular proliferation, and blood perfusion. Methods and Materials: Nude mice bearing Dunning prostate tumors (R3327-AT) were injected with 18F-FDG and pimonidazole, bromodeoxyuridine, and, 1 min before sacrifice, with Hoechst 33342. Selected tumor sections were imaged by phosphor plate autoradiography, while adjacent sections were used to obtain the images of the spatial distribution of Hoechst 33342, pimonidazole, and bromodeoxyuridine. The images were co-registered and analyzed on a pixel-by-pixel basis. Results: Statistical analysis of the data obtained from these tumors demonstrated that 18F-FDG uptake was positively correlated with pimonidazole staining intensity in each data set studied. Correlation of FDG uptake with bromodeoxyuridine staining intensity was always negative. In addition, FDG uptake was always negatively correlated with the staining intensity of Hoechst 33342. Conclusions: For the Dunning prostate tumors studied, FDG uptake was always positively correlated with hypoxia and negatively correlated with both cellular proliferation and blood flow. Therefore, for the tumor model studied, higher FDG uptake is indicative of tumor hypoxia, but neither blood flow nor cellular proliferation. © 2005 Elsevier Inc. |
| Keywords: | nonhuman; positron emission tomography; radiopharmaceuticals; neoplasms; tumor proliferation; cell proliferation; animal cell; mouse; animals; mice; animal experiment; animal model; diagnostic imaging; hypoxia; prostatic neoplasms; blood; correlation analysis; statistical analysis; mice, nude; medical imaging; tumors; prostate tumor; rat; staining; fluorodeoxyglucose f 18; fluorodeoxyglucose f18; radiography; glucose; hoe 33342; pimonidazole; tumor model; cell hypoxia; microenvironment; tracer; blood flow; tumor hypoxia; autoradiography; nitroimidazoles; benzimidazoles; fdg; broxuridine; bromodeoxyuridine; phosphorus; cells; tumor microenvironment; glucose metabolism; blood perfusion; mathematical models; cellular proliferation; coloring agents; pet tumor tracers |
| Journal Title: | International Journal of Radiation Oncology, Biology, Physics |
| Volume: | 62 |
| Issue: | 2 |
| ISSN: | 0360-3016 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2005-06-01 |
| Start Page: | 545 |
| End Page: | 553 |
| Language: | English |
| DOI: | 10.1016/j.ijrobp.2005.02.009 |
| PUBMED: | 15890599 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 65" - "Export Date: 24 October 2012" - "CODEN: IOBPD" - "Source: Scopus" |
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