Past, present and future molecular diagnosis and characterization of human immunodeficiency virus infections Journal Article
| Authors: | Tang, Y. W.; Ou, C. Y. |
| Article Title: | Past, present and future molecular diagnosis and characterization of human immunodeficiency virus infections |
| Abstract: | Substantive and significant advances have been made in the last two decades in the characterization of human immunodeficiency virus (HIV) infections using molecular techniques. These advances include the use of real-time measurements, isothermal amplification, the inclusion of internal quality assurance protocols, device miniaturization and the automation of specimen processing. The result has been a significant increase in the availability of results to a high level of accuracy and quality. Molecular assays are currently widely used for diagnostics, antiretroviral monitoring and drug resistance characterization in developed countries. Simple and cost-effective point-of-care versions are also being vigorously developed with the eventual goal of providing timely healthcare services to patients residing in remote areas and those in resource-constrained countries. In this review, we discuss the evolution of these molecular technologies, not only in the context of the virus, but also in the context of tests focused on human genomics and transcriptomics. © 2012 SSCC. All rights reserved. |
| Keywords: | review; human immunodeficiency virus infection; phenotype; reverse transcription polymerase chain reaction; genotype; enzyme linked immunosorbent assay; cost effectiveness analysis; virus rna; nucleotide sequence; diagnosis; cd4+ t lymphocyte; real time polymerase chain reaction; antiretrovirus agent; cd4 lymphocyte count; virus load; virus genome; hiv; human immunodeficiency virus 1; antiviral resistance; dna polymorphism; molecular diagnosis; gag protein; efavirenz; nucleic acid amplification; nevirapine; maraviroc; antiviral susceptibility |
| Journal Title: | Emerging Microbes & Infections |
| Volume: | 1 |
| ISSN: | 2222-1751 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2012-01-01 |
| Start Page: | e19 |
| Language: | English |
| DOI: | 10.1038/emi.2012.15 |
| PROVIDER: | scopus |
| PMCID: | PMC3630918 |
| PUBMED: | 26038427 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 July 2013" - "Source: Scopus" |
