Pearls and pitfalls of genomics-based microbiome analysis Journal Article
| Authors: | Carlos, N.; Tang, Y. W.; Pei, Z. |
| Article Title: | Pearls and pitfalls of genomics-based microbiome analysis |
| Abstract: | Next generation sequencing (NGS) has supplanted traditional methods for microbiome analyses. Advantages include higher resolution and lower costs. Analyses have evolved from basic 16S rRNA surveys to metagenomic shotgun sequencing analyses. Limitations and obstacles still exist, e.g. 16S bias, bioinformatics bottlenecks, classification accuracies, and genome assembly using short reads. New tools are being developed to improve output and deal with larger amounts of data generated. © 2012 SSCC. All rights reserved. |
| Keywords: | antibiotic agent; gene sequence; sequence analysis; nonhuman; gold standard; genetic analysis; disease association; gene amplification; esophagitis; obesity; diabetes mellitus; genomics; rheumatoid arthritis; asthma; rna 16s; bioinformatics; bacterium culture; bacterium identification; fungus; periodontal disease; pyrosequencing; microbiome; metagenomics; archaeon; prebiotic agent; anthrax; food allergy; microbial genetics; microbial population dynamics; protist |
| Journal Title: | Emerging Microbes & Infections |
| Volume: | 1 |
| ISSN: | 2222-1751 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2012-01-01 |
| Start Page: | e45 |
| Language: | English |
| DOI: | 10.1038/emi.2012.41 |
| PROVIDER: | scopus |
| PMCID: | PMC3634131 |
| PUBMED: | 26038412 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 July 2013" - "Source: Scopus" |
