In vivo bioluminescence imaging and histopathopathologic analysis reveal distinct roles for resident and recruited immune effector cells in defense against invasive aspergillosis Journal Article

Authors: Ibrahim-Granet, O.; Jouvion, G.; Hohl, T. M.; Droin-Bergère, S.; Philippart, F.; Kim, O. Y.; Adib-Conquy, M.; Schwendener, R.; Cavaillon, J. M.; Brock, M.
Article Title: In vivo bioluminescence imaging and histopathopathologic analysis reveal distinct roles for resident and recruited immune effector cells in defense against invasive aspergillosis
Abstract: Background. Invasive aspergillosis (IA) is a major cause of infectious morbidity and mortality in immune compromised patients. Studies on the pathogenesis of IA have been limited by the difficulty to monitor disease progression in real-time. For real-time monitoring of the infection, we recently engineered a bioluminescent A. fumigatus strain. Results. In this study, we demonstrate that bioluminescence imaging can track the progression of IA at different anatomic locations in a murine model of disease that recapitulates the natural route of infection. To define the temporal and functional requirements of distinct innate immune cellular subsets in host defense against respiratory A. fumigatus infection, we examined the development and progression of IA using bioluminescence imaging and histopathologic analysis in mice with four different types of pharmacologic or numeric defects in innate immune function that target resident and recruited phagocyte subsets. While bioluminescence imaging can track the progression and location of invasive disease in vivo, signals can be attenuated by severe inflammation and associated tissue hypoxia. However, especially under non-inflammatory conditions, such as cyclophosphamide treatment, an increasing bioluminescence signal reflects the increasing biomass of alive fungal cells. Conclusions. Imaging studies allowed an in vivo correlation between the onset, peak, and kinetics of hyphal tissue invasion from the lung under conditions of functional or numeric inactivation of phagocytes and sheds light on the germination speed of conidia under the different immunosuppression regimens. Conditions of high inflammation -either mediated by neutrophil influx under corticosteroid treatment or by monocytes recruited during antibody-mediated depletion of neutrophils- were associated with rapid conidial germination and caused an early rise in bioluminescence post-infection. In contrast, 80% alveolar macrophage depletion failed to trigger a bioluminescent signal, consistent with the notion that neutrophil recruitment is essential for early host defense, while alveolar macrophage depletion can be functionally compensated. © 2010 Ibrahim-Granet et al; licensee BioMed Central Ltd.
Keywords: controlled study; unclassified drug; genetics; disease course; histopathology; nonhuman; methodology; mouse; animal; cytology; metabolism; animals; mice; mus; reverse transcription polymerase chain reaction; animal experiment; animal model; inflammation; cyclophosphamide; in vivo study; photoprotein; luminescent proteins; monoclonal antibody; disease model; biosynthesis; immunology; chemistry; disease severity; neutrophil; luminescence; whole body imaging; luminescent measurements; reverse transcriptase polymerase chain reaction; disease progression; lung; murinae; single drug dose; effector cell; innate immunity; neutrophils; real time polymerase chain reaction; monocyte; monocytes; corticosteroid; disease models, animal; microbiology; phagocytosis; immunosuppressive treatment; hypoxemia; cytochemistry; histocytochemistry; bioluminescence; aspergillus fumigatus; aspergillosis; lung aspergillosis; respiratory tract infection; transgenic organism; organisms, genetically modified; immunosuppressive agent; phagocyte; lung alveolus macrophage; macrophages, alveolar; immunosuppression; invasive aspergillosis; dna, fungal; invasive pulmonary aspergillosis; fungal strain; clodronic acid; clodrolip; cortisone acetate; rb 68c5; fungal dna; fungal biomass; fungal cell
Journal Title: BMC Microbiology
Volume: 10
ISSN: 1471-2180
Publisher: Biomed Central Ltd  
Date Published: 2010-04-08
Start Page: 105
Language: English
DOI: 10.1186/1471-2180-10-105
PUBMED: 20377900
PROVIDER: scopus
PMCID: PMC2859869
Notes: --- - "Cited By (since 1996): 3" - "Export Date: 20 April 2011" - "Art. No.: 105" - "Source: Scopus"
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MSK Authors
  1. Tobias Martin Hohl
    76 Hohl