Essential role for neutrophils but not alveolar macrophages at early time points following aspergillus fumigatus infection Journal Article


Authors: Mircescu, M. M.; Lipuma, L. B.; Van Rooijen, N.; Pamer, E. G.; Hohl, T. M.
Article Title: Essential role for neutrophils but not alveolar macrophages at early time points following aspergillus fumigatus infection
Abstract: Alveolar macrophages and neutrophils mediate innate immune defense against the opportunistic fungal pathogen Aspergillus fumigatus and are believed to be essential for host survival following inhalation of fungal spores (conidia). Although alveolar macrophages are postulated to kill inhaled conidia and neutrophils are believed to act against hyphae, the relative contribution of alveolar macrophages and neutrophils to early defense against A. fumigatus remain incompletely denned. To more precisely characterize the contributions of alveolar macrophages and neutrophils in antifungal host defense, we selectively depleted each cell population at different times following pulmonary challenge with conidia. Mice depleted of alveolar macrophages prior to pulmonary A, fumigatus infection recruited neutrophils normally and restricted hyphal tissue invasion. In contrast, neutrophil depletion prior to or within 3 h after infection was associated with high mortality. Neutrophil depletion at later time points, however, was associated with nearly normal survival rates. Our studies suggest that neutrophils, but not alveolar macrophages, provide essential anticonidial defense and that a brief period of influx into the respiratory tree is sufficient to prevent conidial germination and invasive disease. © 2009 by the infectious Diseases Society of America. All rights reserved.
Keywords: controlled study; survival rate; mortality; pathogenesis; nonhuman; mouse; animal; cytology; animals; mice; animal tissue; cell survival; cell function; animal experiment; antifungal agent; cell population; mice, inbred c57bl; physiology; time; time factors; c57bl mouse; immunology; neutrophil; lung; innate immunity; neutrophils; host resistance; microbiology; cell invasion; aspergillus fumigatus; conidium; fungus hyphae; aspergillosis; fungus spore; lung aspergillosis; pulmonary aspergillosis; depletion; germination; inhalation; lung alveolus macrophage; macrophages, alveolar
Journal Title: Journal of Infectious Diseases
Volume: 200
Issue: 4
ISSN: 0022-1899
Publisher: Oxford University Press  
Date Published: 2009-08-15
Start Page: 647
End Page: 656
Language: English
DOI: 10.1086/600380
PUBMED: 19591573
PROVIDER: scopus
PMCID: PMC2745295
DOI/URL:
Notes: --- - "Cited By (since 1996): 10" - "Export Date: 30 November 2010" - "CODEN: JIDIA" - "Source: Scopus"
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MSK Authors
  1. Tobias Martin Hohl
    76 Hohl
  2. Eric Pamer
    277 Pamer
  3. Lauren Beth Lipuma
    14 Lipuma