Protein interaction discovery using parallel analysis of translated ORFs (PLATO) Journal Article
| Authors: | Zhu, J.; Larman, H. B.; Gao, G.; Somwar, R.; Zhang, Z.; Laserson, U.; Ciccia, A.; Pavlova, N.; Church, G.; Zhang, W.; Kesari, S.; Elledge, S. J. |
| Article Title: | Protein interaction discovery using parallel analysis of translated ORFs (PLATO) |
| Abstract: | Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe PLATO, an in vitro method for mapping such interactions by affinity enrichment of a library of full-length open reading frames displayed on ribosomes, followed by massively parallel analysis using DNA sequencing. We demonstrate the broad utility of the method for human proteins by identifying known and previously unidentified interacting partners of LYN kinase, patient autoantibodies, and the small-molecules gefitinib and dasatinib. © 2013 Nature America, Inc. All rights reserved. |
| Keywords: | proteins; protein analysis; protein protein interaction; protein binding; protein interaction; in vitro study; dasatinib; protein p53; gefitinib; protein biosynthesis; dna sequence; protein folding; protein interaction mapping; src-family kinases; dna sequences; paraneoplastic neuropathy; protein kinase lyn; molecules; open reading frame; ribosome; open reading frames; protein sh2; protein a; high-throughput nucleotide sequencing; protein interaction maps; biological analysis; dna sequencing; human proteins; massively parallels; parallel analysis; physical interactions; protein g; protein sh3 |
| Journal Title: | Nature Biotechnology |
| Volume: | 31 |
| Issue: | 4 |
| ISSN: | 1087-0156 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2013-04-01 |
| Start Page: | 331 |
| End Page: | 334 |
| Language: | English |
| DOI: | 10.1038/nbt.2539 |
| PROVIDER: | scopus |
| PUBMED: | 23503679 |
| PMCID: | PMC4110636 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 3 June 2013" - "CODEN: NABIF" - "Source: Scopus" |
