Quantitative, Genome-Wide Analysis of Eukaryotic Replication Initiation and Termination Journal Article

   


Authors: Mcguffee, S. R.; Smith, D. J.; Whitehouse, I.
Article Title: Quantitative, Genome-Wide Analysis of Eukaryotic Replication Initiation and Termination
Abstract: Many fundamental aspects of DNA replication, such as the exact locations where DNA synthesis is initiated and terminated, how frequently origins are used, and how fork progression is influenced by transcription, are poorly understood. Via the deep sequencing of Okazaki fragments, we comprehensively document replication fork directionality throughout the S. cerevisiae genome, which permits the systematic analysis of initiation, origin efficiency, fork progression, and termination. We show that leading-strand initiation preferentially occurs within a nucleosome-free region at replication origins. Using a strain in which late origins can be induced to fire early, we show that replication termination is a largely passive phenomenon that does not rely on cis-acting sequences or replication fork pausing. The replication profile is predominantly determined by the kinetics of origin firing, allowing us to reconstruct chromosome-wide timing profiles from an asynchronous culture. © 2013 Elsevier Inc.
Journal Title: Molecular Cell
Volume: 50
Issue: 1
ISSN: 1097-2765
Publisher: Cell Press  
Date Published: 2013-04-01
Start Page: 123
End Page: 135
Language: English
PROVIDER: scopus
PMCID: PMC3628276
PUBMED: 23562327
DOI: 10.1016/j.molcel.2013.03.004
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84876073691&partnerID=40&md5=049b3c6a522729f7cdf0f561b245cbd4
Notes: --- - "Export Date: 1 May 2013" - "CODEN: MOCEF" - ":doi 10.1016/j.molcel.2013.03.004" - "Source: Scopus"
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MSK Authors
  1. Iestyn Whitehouse
    16 Whitehouse
  2. Sean Robert Mcguffee
    1 Mcguffee
  3. Duncan John Smith
    5 Smith
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