Identification of genes responsive to solar simulated UV radiation in human monocyte-derived dendritic cells Journal Article
| Authors: | de la Fuente, H.; Lamana, A.; Mittelbrunn, M.; Perez-Gala, S.; Gonzalez, S.; García-Diez, A.; Vega, M.; Sanchez-Madrid, F. |
| Article Title: | Identification of genes responsive to solar simulated UV radiation in human monocyte-derived dendritic cells |
| Abstract: | Ultraviolet (UV) irradiation has profound effects on the skin and the systemic immune system. Several effects of UV radiation on Dendritic cells (DCs) functions have been described. However, gene expression changes induced by UV radiation in DCs have not been addressed before. In this report, we irradiated human monocyte-derived DCs with solar-simulated UVA/UVB and analyzed regulated genes on human whole genome arrays. Results were validated by RT-PCR and further analyzed by Gene Set Enrichment Analysis (GSEA). Solar-simulated UV radiation up-regulated expression of genes involved in cellular stress and inflammation, and down-regulated genes involved in chemotaxis, vesicular transport and RNA processing. Twenty four genes were selected for comparison by RT-PCR with similarly treated human primary keratinocytes and human melanocytes. Several genes involved in the regulation of the immune response were differentially regulated in UVA/UVB irradiated human monocyte-derived DCs, such as protein tyrosine phosphatase, receptor type E (PTPRE), thrombospondin-1 (THBS1), inducible costimulator ligand (ICOSL), galectins, Src-like adapter protein (SLA), IL-10 and CCR7. These results indicate that UV-exposure triggers the regulation of a complex gene repertoire involved in human-DC-mediated immune responses. © 2009 de la Fuente et al. |
| Keywords: | controlled study; unclassified drug; human cell; ultraviolet radiation; metabolism; cells, cultured; reverse transcription polymerase chain reaction; gene expression profiling; dendritic cell; melanocyte; interleukin 10; interleukin 1beta; interleukin 8; inflammation; down-regulation; gene product; gene function; drug effect; keratinocyte; radiation exposure; radiation response; immunoregulation; gene expression regulation; genome analysis; dendritic cells; chemotaxis; cell culture; gene identification; human genome; binding protein; down regulation; calcium binding protein; upregulation; cycline; monocyte; monocytes; up-regulation; c terminal binding protein 2; calcium binding protein 39; chemokine receptor ccr7; cxcl2 chemokine; galectin; growth differentiation factor 15; human immunodeficiency virus type 1 enhancer binding protein; inducible t cell costimulator ligand; interleukin 1alpha; microphthalmia associated transcription factor; myosin v; myosin vb; oxidoreductase; polo like kinase 2; protein tyrosine phosphatase receptor type e; src like adapter protein; tensin; thrombospondin 1; tripartite motif containing 9 protein; ubiquitin protein ligase e3; ubiquitin protein ligase e3a; ww domain containing oxidoreductase; cell transport; cellular stress response; immune response gene; monocyte derived dendritic cell; rna processing; ultraviolet a radiation; ultraviolet b radiation; ultraviolet rays |
| Journal Title: | PLoS ONE |
| Volume: | 4 |
| Issue: | 8 |
| ISSN: | 1932-6203 |
| Publisher: | Public Library of Science |
| Date Published: | 2009-08-26 |
| Start Page: | e6735 |
| Language: | English |
| DOI: | 10.1371/journal.pone.0006735 |
| PUBMED: | 19707549 |
| PROVIDER: | scopus |
| PMCID: | PMC2727914 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 30 November 2010" - "Art. No.: e6735" - "Source: Scopus" |
