Regulation of Microtubule Stability and Organization by Mammalian Par3 in Specifying Neuronal Polarity Journal Article
| Authors: | Chen, S.; Chen, J.; Shi, H.; Wei, M.; Castaneda-Castellanos, D. R.; Bultje, R. S.; Pei, X.; Kriegstein, A. R.; Zhang, M.; Shi, S. H. |
| Article Title: | Regulation of Microtubule Stability and Organization by Mammalian Par3 in Specifying Neuronal Polarity |
| Abstract: | Polarization of mammalian neurons with a specified axon requires precise regulation of microtubule and actin dynamics in the developing neurites. Here we show that mammalian partition defective 3 (mPar3), a key component of the Par polarity complex that regulates the polarization of many cell types including neurons, directly regulates microtubule stability and organization. The N-terminal portion of mPar3 exhibits strong microtubule binding, bundling, and stabilization activity, which can be suppressed by its C-terminal portion via an intramolecular interaction. Interestingly, the intermolecular oligomerization of mPar3 is able to relieve the intramolecular interaction and thereby promote microtubule bundling and stabilization. Furthermore, disruption of this microtubule regulatory activity of mPar3 impairs its function in axon specification. Together, these results demonstrate a role for mPar3 in directly regulating microtubule organization that is crucial for neuronal polarization. Chen et al. report that mammalian partition defective 3 (mPar3), a key component of the Par polarity complex, functions as a conformation-dependent microtubule-associated protein that directly binds, bundles, and stabilizes microtubules. This microtubule regulatory activity of mPar3 is essential for its function in regulating axon specification and neuronal polarization. © 2013 Elsevier Inc.. |
| Journal Title: | Developmental Cell |
| Volume: | 24 |
| Issue: | 1 |
| ISSN: | 1534-5807 |
| Publisher: | Cell Press |
| Date Published: | 2013-01-14 |
| Start Page: | 26 |
| End Page: | 40 |
| Language: | English |
| DOI: | 10.1016/j.devcel.2012.11.014 |
| PROVIDER: | scopus |
| PMCID: | PMC3549028 |
| PUBMED: | 23273878 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 February 2013" - "CODEN: DCEEB" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work