Sensitive, accurate and simple liquid chromatography-tandem mass spectrometric method for the quantitation of amphotericin B in human or minipig plasma Journal Article
| Authors: | Qin, W.; Tao, H.; Chen, Y.; Chen, Z.; Wu, N. |
| Article Title: | Sensitive, accurate and simple liquid chromatography-tandem mass spectrometric method for the quantitation of amphotericin B in human or minipig plasma |
| Abstract: | Amphotericin B (AMB) is still the standard care for systemic fungal infections. This paper describes a sensitive, accurate and simple liquid chromatography-tandem mass spectrometry method to quantify AMB in human or minipig plasma. Samples were prepared through protein precipitation by adding methanol-acetonitrile (1:3, v/v) to either human or minipig plasma. High-performance liquid chromatography separation was conducted on a 10-cm Gemini C18 column with a 7-min gradient of mobile phase comprised of buffer A (0.1% formic acid aqueous solution) and buffer B (methanol-acetonitrile, 2:3, v/v). AMB was detected through multiple reaction monitoring (MRM) with a mass transition of 924.60 →743.30 and the internal standard paclitaxel was detected through MRM with a mass transition of 854.30 →286.10. The method had a linear range between 5 and 2500 ng/mL with lower limit of quantitation of 3 ng/mL. The overall recovery was 113±4.06% in human plasma and 94.8±7.38% in minipig plasma. The method has been validated and applied for AMB pharmacokinetic study in both human and minipig plasma. © The Author [2012]. Published by Oxford University Press. All rights reserved. |
| Keywords: | methodology; reproducibility; linear models; reproducibility of results; animal; animals; blood; tandem mass spectrometry; high performance liquid chromatography; chromatography, high pressure liquid; statistical model; swine; drug stability; limit of detection; minipig; amphotericin b; swine, miniature |
| Journal Title: | Journal of Chromatographic Science |
| Volume: | 50 |
| Issue: | 7 |
| ISSN: | 0021-9665 |
| Publisher: | Oxford University Press |
| Date Published: | 2012-01-01 |
| Start Page: | 636 |
| End Page: | 643 |
| Language: | English |
| DOI: | 10.1093/chromsci/bms049 |
| PUBMED: | 22562820 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Export Date: 28 January 2013" - "CODEN: JCHSB" - "Source: Scopus" |
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