The nuclear export receptor XPO-1 supports primary miRNA processing in C. elegans and Drosophila Journal Article

   

Authors:	Büssing, I.; Yang, J. S.; Lai, E. C.; Grohans, H.
Article Title:	The nuclear export receptor XPO-1 supports primary miRNA processing in C. elegans and Drosophila
Abstract:	MicroRNA (miRNA) biogenesis proceeds from a primary transcript (pri-miRNA) through the pre-miRNA into the mature miRNA. Here, we identify a role of the Caenorhabditis elegans nuclear export receptor XPO-1 and the cap-binding proteins CBP-20/NCBP-2 and CBP-80/NCBP-1 in this process. The RNA-mediated interference of any of these genes causes retarded heterochronic phenotypes similar to those observed for animals with mutations in the let-7 miRNA or core miRNA machinery genes. Moreover, pre-and mature miRNAs become depleted, whereas primary miRNA transcripts accumulate. An involvement of XPO-1 in miRNA biogenesis is conserved in Drosophila, in which knockdown of Embargoed/XPO-1 or its chemical inhibition through leptomycin B causes pri-miRNA accumulation. Our findings demonstrate that XPO-1/Emb promotes the pri-miRNA-to-pre-miRNA processing and we propose that this function involves intranuclear transport and/or nuclear export of primary miRNAs. © 2010 European Molecular Biology Organization.
Keywords:	controlled study; protein expression; unclassified drug; gene mutation; nonhuman; phenotype; animals; microrna; protein depletion; drosophila; rna interference; animalia; active transport, cell nucleus; caenorhabditis elegans; micrornas; rna processing; rna transcription; biogenesis; cap binding protein; drosophila protein; receptors, cytoplasmic and nuclear; cbp20/ncbp-2; cbp80/ncbp-1; microrna biogenesis; nuclear export; xpo1/crm1/embargoed; cap binding protein 20; cap binding protein 80; exportin 1; leptomycin b; microrna let 7; protein embargoed; karyopherins; nuclear cap-binding protein complex; rna cap-binding proteins
Journal Title:	EMBO Journal
Volume:	29
Issue:	11
ISSN:	0261-4189
Publisher:	Wiley Blackwell
Date Published:	2010-06-02
Start Page:	1830
End Page:	1839
Language:	English
DOI:	10.1038/emboj.2010.82
PUBMED:	20436454
PROVIDER:	scopus
PMCID:	PMC2885935
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-77953123717&partnerID=40&md5=2ccc2da9e80a8a8c3814ba3beaaedfc6
Notes:	--- - "Cited By (since 1996): 6" - "Export Date: 20 April 2011" - "CODEN: EMJOD" - "Source: Scopus"

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