Crystal structure of the calcium release-activated calcium channel orai Journal Article

   


Authors: Hou, X.; Pedi, L.; Diver, M. M.; Long, S. B.
Article Title: Crystal structure of the calcium release-activated calcium channel orai
Abstract: The plasma membrane protein Orai forms the pore of the calcium release-activated calcium (CRAC) channel and generates sustained cytosolic calcium signals when triggered by depletion of calcium from the endoplasmic reticulum. The crystal structure of Orai from Drosophila melanogaster, determined at 3.35 angstrom resolution, reveals that the calcium channel is composed of a hexameric assembly of Orai subunits arranged around a central ion pore. The pore traverses the membrane and extends into the cytosol. A ring of glutamate residues on its extracellular side forms the selectivity filter. A basic region near the intracellular side can bind anions that may stabilize the closed state. The architecture of the channel differs markedly from other ion channels and gives insight into the principles of selective calcium permeation and gating.
Keywords: nonhuman; animals; protein assembly; protein; protein binding; protein stability; membrane proteins; calcium; endoplasmic reticulum; cytoplasm; crystal structure; crystallography, x-ray; protein structure, tertiary; binding sites; fly; drosophila melanogaster; porosity; protein subunit; biochemistry; glutamic acid; drosophila proteins; membrane transport; protein structure, secondary; plasma; cytosol; permeability; ion; membrane; calcium transport; calcium signaling; calcium channel; calcium release activated calcium channel; calcium channels
Journal Title: Science
Volume: 338
Issue: 6112
ISSN: 0036-8075
Publisher: American Association for the Advancement of Science  
Date Published: 2012-12-07
Start Page: 1308
End Page: 1313
Language: English
DOI: 10.1126/science.1228757
PROVIDER: scopus
PUBMED: 23180775
PMCID: PMC3695727
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84870655957&partnerID=40&md5=0c2ed72f9129adc040c4f292727fc1e6
Notes: --- - "Export Date: 2 January 2013" - "CODEN: SCIEA" - "Source: Scopus"
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MSK Authors
  1. Xiaowei Hou
    7 Hou
  2. Stephen Barstow Long
    34 Long
  3. Leanne Pedi
    4 Pedi
  4. Melinda M Diver
    7 Diver
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