Cryo-EM structures of fungal and metazoan mitochondrial calcium uniporters Journal Article
| Authors: | Baradaran, R.; Wang, C.; Siliciano, A. F.; Long, S. B. |
| Article Title: | Cryo-EM structures of fungal and metazoan mitochondrial calcium uniporters |
| Abstract: | The mitochondrial calcium uniporter (MCU) is a highly selective calcium channel and a major route of calcium entry into mitochondria. How the channel catalyses ion permeation and achieves ion selectivity are not well understood, partly because MCU is thought to have a distinct architecture in comparison to other cellular channels. Here we report cryo-electron microscopy reconstructions of MCU channels from zebrafish and Cyphellophora europaea at 8.5 angstrom and 3.2 angstrom resolutions, respectively. In contrast to a previous report of pentameric stoichiometry for MCU, both channels are tetramers. The atomic model of C. europaea MCU shows that a conserved WDXXEP signature sequence forms the selectivity filter, in which calcium ions are arranged in single file. Coiled-coil legs connect the pore to N-terminal domains in the mitochondrial matrix. In C. europaea MCU, the N-terminal domains assemble as a dimer of dimers; in zebrafish MCU, they form an asymmetric crescent. The structures define principles that underlie ion permeation and calcium selectivity in this unusual channel. |
| Keywords: | antibodies; system; crystal-structure; essential component; membrane-proteins; potassium channel; ion-channel; k+ channel; ca2+ uptake; mcur1 |
| Journal Title: | Nature |
| Volume: | 559 |
| Issue: | 7715 |
| ISSN: | 0028-0836 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2018-07-26 |
| Start Page: | 580 |
| End Page: | 584 |
| Language: | English |
| ACCESSION: | WOS:000439850800049 |
| DOI: | 10.1038/s41586-018-0331-8 |
| PROVIDER: | wos |
| PUBMED: | 29995857 |
| PMCID: | PMC6336196 |
| Notes: | Article -- Source: Wos |
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