Serological identification of breast cancer-related antigens from a Saccharomyces cerevisiae surface display library Journal Article

  


Authors: Wadle, A.; Mischo, A.; Imig, J.; Wüllner, B.; Hensel, D.; Wätzig, K.; Neumann, F.; Kubuschok, B.; Schmidt, W.; Old, L. J.; Pfreundschuh, M.; Renner, C.
Article Title: Serological identification of breast cancer-related antigens from a Saccharomyces cerevisiae surface display library
Abstract: A yeast cell surface display technology was used for the isolation and characterization of tumor antigens recognised by autologous or allogeneic breast cancer serum. More than 100 clones recognized by patient serum were isolated using high-through-put fluorescence activated cell sorting. Combined serological and sequence analysis confirmed that a number of proteins known to be overexpressed in breast cancer tissue could be detected. A recently identified small breast epithelial mucin almost exclusively expressed in mammary gland tissue was isolated as a mutated protein variant. Subsequent serological analysis using the yeast expression system for the wild-type and mutant form showed a strong recognition by patient sera, whereas no significant recognition was observed for the respective prokaryotically expressed proteins. The small breast epithelial mucin is present to a large extent in a membrane bound format and might be used for tumor targeting strategies. © 2005 Wiley-Liss, Inc.
Keywords: controlled study; human tissue; sequence analysis; flow cytometry; gene overexpression; reverse transcription polymerase chain reaction; breast cancer; membrane proteins; immunofluorescence; gene library; breast neoplasms; wild type; tumor antigen; enzyme linked immunosorbent assay; collagen type 1; rna binding protein; molecular cloning; cloning, molecular; gene expression regulation, neoplastic; amino acid sequence; molecular sequence data; sequence homology, amino acid; antigens, neoplasm; saccharomyces cerevisiae; tumor antigens; nucleotide sequence; antigen recognition; western blotting; breast epithelium; base sequence; glyceraldehyde 3 phosphate dehydrogenase; enzyme-linked immunosorbent assay; sequence homology, nucleic acid; ku antigen; hla a antigen; fluorescence activated cell sorting; complementary dna; antigens, surface; ribosome protein; mammary glands, human; dna, complementary; plasmid dna; mucin; dystrobrevin; mucins; clathrin; cytochrome c oxidase; reduced nicotinamide adenine dinucleotide dehydrogenase; fructose bisphosphate aldolase; initiation factor 2; protein swi; dna library; protein disulfide isomerase; ran protein; cdna expression library; yeast surface display; proton transporting adenosine triphosphate synthase; serologic tests; serological screening; deoxyhypusine synthase; parathymosin; selenoprotein; thymosin beta10; ubiquitin c
Journal Title: International Journal of Cancer
Volume: 117
Issue: 1
ISSN: 0020-7136
Publisher: John Wiley & Sons  
Date Published: 2005-10-20
Start Page: 104
End Page: 113
Language: English
DOI: 10.1002/ijc.21147
PUBMED: 15880534
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-24344435109&partnerID=40&md5=7e0528dc5fb80c1d5d02f0e6c9292d9b
Notes: --- - "Cited By (since 1996): 25" - "Export Date: 24 October 2012" - "CODEN: IJCNA" - "Molecular Sequence Numbers: GENBANK: AB051293, AF030235, AF318324, AF537132, AL390147, AY339576, AY651259, BC014002, BC016800, BC051312, BT006893, D32129, L05093, NM_000088, NM_000990, NM_000991, NM_001469, NM_002824, NM_004859, NM_005105, NM_005336, NM_006356, NM_006830, NM_015904, NM_021103, NM_033301, NM_058173, NM_173705, U26266, XM_055013, XM_290535, Y12712, Z49835;" - "Source: Scopus"
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  1. Lloyd J Old
    593 Old
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