Identification of B cell epitopes recognized by antibodies specific for the tumor antigen NY-ESO-1 in cancer patients with spontaneous immune responses Journal Article
| Authors: | Valmori, D.; Souleimanian, N. E.; Hesdorffer, C. S.; Ritter, G.; Old, L. J.; Ayyoub, M. |
| Article Title: | Identification of B cell epitopes recognized by antibodies specific for the tumor antigen NY-ESO-1 in cancer patients with spontaneous immune responses |
| Abstract: | Expression of the germ line antigen NY-ESO-1 in adult somatic tissues other than testis is strictly found in association with cancer. Patients bearing NY-ESO-1 expressing tumors often develop integrated specific immune responses to the antigen, encompassing T cell and antibody responses. Hence, detection of NY-ESO-1 specific antibody responses can be considered as a cancer biomarker of great interest. Here, we used synthetic peptides spanning the sequence of the NY-ESO-1 protein to assess antibody responses in cancer patients. This approach allowed the identification of peptides containing linear B cell epitopes. Some peptides were recognized by the majority of seropositive patients thus identifying several distinct regions of the protein containing frequently recognized B cell epitopes. The results of this study provide the first appraisal of the diversity of naturally-occurring NY-ESO-1 specific antibodies and could be instrumental in the monitoring of therapy-induced antibody responses in cancer patients receiving NY-ESO-1-based vaccines. © 2005 Elsevier Inc. All rights reserved. |
| Keywords: | controlled study; unclassified drug; genetics; cancer patient; molecular genetics; methodology; neoplasm; neoplasms; protein analysis; tumor markers, biological; membrane proteins; peptide; tumor antigen; tumor marker; enzyme linked immunosorbent assay; blotting, western; immunology; immune response; amino acid sequence; molecular sequence data; antigens, neoplasm; molecular recognition; antibody response; recombinant proteins; epitope; ctag1b protein, human; membrane protein; recombinant protein; western blotting; peptides; tumor immunity; antibody specificity; antibodies; enzyme-linked immunosorbent assay; epitopes, b-lymphocyte; serology; polyacrylamide gel electrophoresis; electrophoresis, polyacrylamide gel; protein antibody; synthetic peptide; b lymphocyte antigen; ny eso 1 antibody; b cell epitopes; protein ny eso 1; serologic tests |
| Journal Title: | Clinical Immunology |
| Volume: | 117 |
| Issue: | 1 |
| ISSN: | 1521-6616 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2005-10-01 |
| Start Page: | 24 |
| End Page: | 30 |
| Language: | English |
| DOI: | 10.1016/j.clim.2005.05.003 |
| PUBMED: | 15994128 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 12" - "Export Date: 24 October 2012" - "CODEN: CLIIF" - "Source: Scopus" |
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