β-Microseminoprotein binds CRISP-3 in human seminal plasma Journal Article
| Authors: | Udby, L.; Lundwall, Å; Johnsen, A. H.; Fernlund, P.; Valtonen-André, C.; Blom, A. M.; Lilja, H.; Borregaard, N.; Kjeldsen, L.; Bjartell, A. |
| Article Title: | β-Microseminoprotein binds CRISP-3 in human seminal plasma |
| Abstract: | β-Microseminoprotein (MSP) and cysteine-rich secretory protein 3 (CRISP-3) are abundant constituents of human seminal plasma. Immunoprecipitation and gel filtration of seminal plasma proteins combined with examination of the proteins in their pure form showed that MSP and CRISP-3 form stable, non-covalent complexes. CRISP-3 binds MSP with very high affinity, as evidenced by surface plasmon resonance. Due to far higher abundance of MSP in prostatic fluid, it manifests large overcapacity for CRISP-3 binding. Structural similarity with an MSP-binding protein from blood plasma suggests that CRISP-3 binds MSP through its aminoterminal SCP-domain. © 2005 Elsevier Inc. All rights reserved. |
| Keywords: | controlled study; protein expression; unclassified drug; nonhuman; binding affinity; mass spectrometry; protein analysis; animal cell; carboxy terminal sequence; protein binding; prostate cancer; amino terminal sequence; kinetics; immunoprecipitation; protein structure; surface plasmon resonance; semen analysis; seminal plasma; psp94; beta microseminoprotein; cysteine rich secretory protein 3; prostatic secretory proteins; seminal plasma proteins; covalent bond; semen; secretory protein; gel filtration; protein complex; salivary proteins; β-microseminoprotein; cysteine-rich secretory protein; scp-domain |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 333 |
| Issue: | 2 |
| ISSN: | 0006-291X |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2005-07-29 |
| Start Page: | 555 |
| End Page: | 561 |
| Language: | English |
| DOI: | 10.1016/j.bbrc.2005.05.139 |
| PUBMED: | 15950934 |
| PROVIDER: | scopus |
| PMCID: | PMC1939934 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 33" - "Export Date: 24 October 2012" - "CODEN: BBRCA" - "Source: Scopus" |
