Fulminant experimental autoimmune encephalomyelitis induced by retrovirally mediated TCR gene transfer Journal Article
| Authors: | Stolzer, A. L.; Sadelain, M.; Sant'angelo, D. B. |
| Article Title: | Fulminant experimental autoimmune encephalomyelitis induced by retrovirally mediated TCR gene transfer |
| Abstract: | Although some efforts have been made to direct the antigen specificity of developing T cells by retroviral mediated expression of known TCR, it is not clear if the resultant T cells are fully functional. In this study retroviral gene transfer technology was used to introduce a cDNA encoding the TCR from a known encephalitogenic T cell into the bone marrow of mice. Activated T cells expressing this TCR, which is specific for the Ac1-11 peptide from myelin basic protein presented by I-Au, cause rapid onset of experimental autoimmune encephalomyelitis (EAE). This enabled us to use the onset and progression of the disease as a direct measure of effector functions of T cells generated by this method. The data presented here show that recipients of bone marrow retrovirally transduced with this TCR rapidly develop full-blown EAE that results in paralysis. Therefore, retroviral TCR delivery into the bone marrow supports the development of T cells into fully functional effector cells. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| Keywords: | controlled study; protein expression; disease course; nonhuman; methodology; t lymphocyte; t-lymphocytes; myelin basic protein; animal cell; mouse; animals; mice; animal tissue; bone marrow; spleen; animal experiment; animal model; gene transfer; transduction, genetic; t lymphocyte receptor; thymus; receptors, antigen, t-cell; data analysis; retrovirus vector; effector cell; genetic code; t lymphocyte activation; complementary dna; lymphocyte function; retroviridae; paralysis; allergic encephalomyelitis; recipient; tcr; interleukin-4; gene technology; myelin basic proteins; encephalomyelitis, autoimmune, experimental; interferon type ii; gene transfer, horizontal; eac; retroviral gene transfer; t lymphocyte receptor gene |
| Journal Title: | European Journal of Immunology |
| Volume: | 35 |
| Issue: | 6 |
| ISSN: | 0014-2980 |
| Publisher: | Wiley V C H Verlag Gmbh |
| Date Published: | 2005-06-01 |
| Start Page: | 1822 |
| End Page: | 1830 |
| Language: | English |
| DOI: | 10.1002/eji.200526123 |
| PUBMED: | 15909313 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 4" - "Export Date: 24 October 2012" - "CODEN: EJIMA" - "Source: Scopus" |
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