Assembly of the N-cadherin complex during synapse formation involves uncoupling of p120-catenin and association with presenilin 1 Journal Article
| Authors: | Rubio, M. E.; Curcio, C.; Chauvet, N.; Bruses, J. L. |
| Article Title: | Assembly of the N-cadherin complex during synapse formation involves uncoupling of p120-catenin and association with presenilin 1 |
| Abstract: | N-cadherin is an adhesion receptor that participates in both interaction between immature pre- and postsynaptic neurons and in the stabilization and function of matured neuron-neuron synapses. To better understand how the N-cadherin complex contributes to synapse formation, we examined its distribution and composition during synapse formation in the chick ciliary neurons. It was found that at early phases of synaptogenesis, N-cadherin is distributed in small clusters on the cell surface and primarily associates with p120-catenin and β-catenin. In contrast, as synaptic contacts matured, larger N-cadherin clusters were found localized adjacent to the active zone and associated with PS1 and γ-catenin, while p120- and β-catenin were dispersed among other cell regions, including axons. As it is known that PS1 binds γ-catenin and that uncoupled p120-catenin can alter the cytoskeleton via its effect on Rho GTPases, these changes in the molecular composition of the N-cadherin complex (represented by the uncoupling of p120-catenin and association with PS1) may correspond to distinct functional states of the complex involved in synaptic maturation. © 2005 Elsevier Inc. All rights reserved. |
| Keywords: | controlled study; nonhuman; protein localization; animal cell; animals; animal tissue; cell function; complex formation; cluster analysis; cell maturation; embryo; protein protein interaction; protein binding; membrane proteins; cell differentiation; neurons; transfection; nerve fiber; nerve cell adhesion molecule; phosphoproteins; cellular distribution; green fluorescent proteins; cell adhesion molecules; chemical structure; trans-activators; beta catenin; cadherins; cytoskeleton; nerve cell; brain region; rho guanine nucleotide binding protein; genetic linkage; presenilin 1; presenilin-1; synapses; cytoskeletal proteins; chick embryo; chickens; cell surface; synapse; cho cells; cricetinae; cell composition; chicken; plakoglobin; synaptogenesis; catenin; protein p120; ciliary ganglion; desmoplakins; gamma catenin; microscopy, immunoelectron |
| Journal Title: | Molecular and Cellular Neuroscience |
| Volume: | 30 |
| Issue: | 1 |
| ISSN: | 1044-7431 |
| Publisher: | Academic Press Inc., Elsevier Science |
| Date Published: | 2005-09-01 |
| Start Page: | 118 |
| End Page: | 130 |
| Language: | English |
| DOI: | 10.1016/j.mcn.2005.06.005 |
| PUBMED: | 16046145 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 17" - "Export Date: 24 October 2012" - "CODEN: MOCNE" - "Source: Scopus" |
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