Protection of cytidine deaminase gene gainst toxicity of high-dose chemotherapy in mice Journal Article

  


Authors: Lu, P.; Wang, Y. L.; Jin, F.; Chen, B.; Yao, F.; Wang, S. B.; Chen, J. Q.; Xu, H. M.; Zhao, S. C.
Article Title: Protection of cytidine deaminase gene gainst toxicity of high-dose chemotherapy in mice
Abstract: AIM: To observe the tolerance to high-dose cytarabine (Ara-C) in mice after the cytidine deaminase (CD) gene is transfected into mouse bone marrow cells, and to explore the feasibility of chemotherapy combined with the tolerance of myelosuppression. METHODS: Human cytidine deaminase gene was transfected into mouse bone marrow cells by retroviral vector. Then the colony-forming unit granulocyte-macrophage (CFU-GM) was observed in the cells of marrow donor and acceptor mice treated with Ara-C. DNA was extracted from the cells and the drug-resistant genes were detected by polymerase chain reaction (PCR). The blood cell count, weight and survival rate of the mice treated with Ara-C were analyzed. RESULTS: Drug-resistant colonies appeared both in the bone marrow cells of donor and acceptor mice treated with Ara-C, and the CFU-GMs were 52% and 54% respectively, which were significantly higher than those of the controls (χ 2 = 124.62, 126.26; both P<0.01). The survival rate was significantly higher in CD-transfected mice as compared with that in the controls (χ 2 = 7.42, P<0.01), and the blood cell count and body weight decreased less and recovered sooner. CD gene was expressed in the bone marrow cells of transfected mice. CONCLUSION: Drug-resistant gene can not only integrate and express in mouse bone marrow cells, but also promote the tolerance to high-dose Ara-C.
Keywords: controlled study; survival rate; drug tolerability; nonhuman; cytarabine; drug megadose; polymerase chain reaction; animal cell; mouse; gene expression; bone marrow suppression; body weight; feasibility study; cytidine deaminase; genetic transfection; gene therapy; retrovirus vector; toxicity testing; bone marrow cell; blood cell count; dna extraction; colony forming unit gm; organ donor
Journal Title: World Chinese Journal of Digestology
Volume: 13
Issue: 14
ISSN: 1009-3079
Publisher: WJC Press  
Date Published: 2005-07-28
Start Page: 1705
End Page: 1708
Language: Chinese
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-23644454202&partnerID=40&md5=a76956f40386c094ad7ca740fdd8345e
Notes: --- - "Export Date: 24 October 2012" - "CODEN: SHXZF" - "Source: Scopus"