The crystal structure of human CD1d with and without α-galactosylceramide Journal Article
| Authors: | Koch, M.; Stronge, V. S.; Shepherd, D.; Gadola, S. D.; Mathew, B.; Ritter, G.; Fersht, A. R.; Besra, G. S.; Schmidt, R. R.; Jones, E. Y.; Cerundolo, V. |
| Article Title: | The crystal structure of human CD1d with and without α-galactosylceramide |
| Abstract: | The glycolipid α-galactosylceramide binds with high affinity to CD1d and stimulates natural killer T cells. Here we report the crystal structure of human CD1d in complex with synthetic α-galactosylceramide at a resolution of 3.0 Å. The structure shows a tightly fit lipid in the CD1d binding groove, with the sphingosine chain bound in the C′ pocket and the longer acyl chain anchored in the A′ pocket. We also present the CD1d structure without lipid, which has a more open conformation of the binding groove, suggesting a dual conformation of CD1d in which the 'open' conformation is more able to load lipids. These structures provide clues as to how CD1 molecules load glycolipids as well as data to guide the design of new therapeutic agents. © 2005 Nature Publishing Group. |
| Keywords: | controlled study; human cell; nonhuman; binding affinity; protein conformation; mouse; lipid; protein binding; crystal structure; protein structure; sphingosine; structure analysis; alpha galactosylceramide; cd1d antigen; natural killer t cell; glycolipid; human versus animal comparison |
| Journal Title: | Nature Immunology |
| Volume: | 6 |
| Issue: | 8 |
| ISSN: | 1529-2908 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2005-08-01 |
| Start Page: | 819 |
| End Page: | 826 |
| Language: | English |
| DOI: | 10.1038/ni1225 |
| PROVIDER: | scopus |
| PUBMED: | 16007090 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 183" - "Export Date: 24 October 2012" - "CODEN: NIAMC" - "Source: Scopus" |
