Antibody response to GD3 ganglioside is independent of NKT cells Journal Article
| Authors: | Park, J. E.; Lu, S. X.; Wu, D. Y.; Prendes, M.; Chapman, P. B. |
| Article Title: | Antibody response to GD3 ganglioside is independent of NKT cells |
| Abstract: | Background: NKT cells recognize glycolipids presented by CD1d on antigen-presenting cells (APC) and have been largely characterized by their ability to be activated by α-galactosylceramide, a glycolipid not expressed on mammalian cells. We have shown previously that GD3 can be cross-presented by CD1d to NKT cells and is the first tumor-derived glycolipid recognized by NKT cells. But the ability of NKT cells to modulate B-cell responses to tumor glycolipids that are themselves recognized by NKT cells has not been explored. Methods: We tested whether NKT cells are required for antibody (Ab) responses to GD3. We immunized wild-type mice, mice deficient in invariant chain NKT cells (iNKT cells) and mice deficient in total NKT cells against GD3. Ab titer against GD3 was measured by ELISA. Results:We found the IgM and IgG responses against GD3 were similar among the three strains of mice, including the IgG isotypes induced. Pre-expanded NKT cells to GD3 did not affect the anti-GD3 Ab response. Discussion: We conclude that Ab responses to GD3 are independent of NKT cells and that strategies to manipulate NKT cells in vivo are not likely to enhance the anti-GD3 Ab response induced by vaccines. |
| Keywords: | controlled study; nonhuman; animal cell; mouse; animals; mice; melanoma; animal experiment; wild type; enzyme linked immunosorbent assay; mice, inbred c57bl; immunoglobulin g; antibody response; killer cells, natural; immunomodulation; immunoglobulin g1; immunoglobulin g3; c57bl 6 mouse; immunoglobulin class switching; enzyme-linked immunosorbent assay; immunoglobulin g2a; antigen presenting cell; t lymphocyte activation; ganglioside gd3; antibody formation; antibody titer; immunoglobulin m; cd1d antigen; natural killer t cell; immunization; gangliosides; cross presentation; gd3 ganglioside; cd1d; nkt cell; immunoglobulin g2b |
| Journal Title: | Cytotherapy |
| Volume: | 10 |
| Issue: | 1 |
| ISSN: | 1465-3249 |
| Publisher: | Elsevier Science Ltd. |
| Date Published: | 2008-01-01 |
| Start Page: | 38 |
| End Page: | 44 |
| Language: | English |
| DOI: | 10.1080/14653240701762380 |
| PUBMED: | 18202973 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Export Date: 17 November 2011" - "CODEN: CYTRF" - "Source: Scopus" |
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