Antibody response to GD3 ganglioside is independent of NKT cells Journal Article


Authors: Park, J. E.; Lu, S. X.; Wu, D. Y.; Prendes, M.; Chapman, P. B.
Article Title: Antibody response to GD3 ganglioside is independent of NKT cells
Abstract: Background: NKT cells recognize glycolipids presented by CD1d on antigen-presenting cells (APC) and have been largely characterized by their ability to be activated by α-galactosylceramide, a glycolipid not expressed on mammalian cells. We have shown previously that GD3 can be cross-presented by CD1d to NKT cells and is the first tumor-derived glycolipid recognized by NKT cells. But the ability of NKT cells to modulate B-cell responses to tumor glycolipids that are themselves recognized by NKT cells has not been explored. Methods: We tested whether NKT cells are required for antibody (Ab) responses to GD3. We immunized wild-type mice, mice deficient in invariant chain NKT cells (iNKT cells) and mice deficient in total NKT cells against GD3. Ab titer against GD3 was measured by ELISA. Results:We found the IgM and IgG responses against GD3 were similar among the three strains of mice, including the IgG isotypes induced. Pre-expanded NKT cells to GD3 did not affect the anti-GD3 Ab response. Discussion: We conclude that Ab responses to GD3 are independent of NKT cells and that strategies to manipulate NKT cells in vivo are not likely to enhance the anti-GD3 Ab response induced by vaccines.
Keywords: controlled study; nonhuman; animal cell; mouse; animals; mice; melanoma; animal experiment; wild type; enzyme linked immunosorbent assay; mice, inbred c57bl; immunoglobulin g; antibody response; killer cells, natural; immunomodulation; immunoglobulin g1; immunoglobulin g3; c57bl 6 mouse; immunoglobulin class switching; enzyme-linked immunosorbent assay; immunoglobulin g2a; antigen presenting cell; t lymphocyte activation; ganglioside gd3; antibody formation; antibody titer; immunoglobulin m; cd1d antigen; natural killer t cell; immunization; gangliosides; cross presentation; gd3 ganglioside; cd1d; nkt cell; immunoglobulin g2b
Journal Title: Cytotherapy
Volume: 10
Issue: 1
ISSN: 1465-3249
Publisher: Elsevier Science Ltd.  
Date Published: 2008-01-01
Start Page: 38
End Page: 44
Language: English
DOI: 10.1080/14653240701762380
PUBMED: 18202973
PROVIDER: scopus
DOI/URL:
Notes: --- - "Export Date: 17 November 2011" - "CODEN: CYTRF" - "Source: Scopus"
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MSK Authors
  1. Yue Wu
    8 Wu
  2. Paul Chapman
    308 Chapman
  3. Jun-Eui Park
    5 Park
  4. Xing-Gu Lu
    5 Lu