Stimulation of T lymphocyte proliferation by monoclonal antibodies against G(D3) ganglioside Journal Article
| Authors: | Welte, K.; Miller, G.; Chapman, P. B.; Yuasa, H.; Natoli, E.; Kunicka, J. E.; Cordon-Cardo, C.; Buhrer, C.; Old, L. J.; Houghton, A. N. |
| Article Title: | Stimulation of T lymphocyte proliferation by monoclonal antibodies against G(D3) ganglioside |
| Abstract: | Cell-surface gangliosides are presumed to play a role in cell growth and differentiation. With the use of monoclonal antibodies directed against G(D3), a disialoganglioside expressed predominantly by cells of neuroectodermal origin, we have found that G(D3) is expressed by a subpopulation of cells of the immune system including: 1) fetal thymocytes in subcortical regions and near vessels, 2) lymph node lymphocytes in interfollicular areas and near vessels, and 3) a small subset of T cells in the peripheral blood. Mouse monoclonal antibodies (two IgGs, one IgM, and F(ab')2 fragments) reacting with G(D3) were found to stimulate proliferation of T cells derived from peripheral blood. Proliferation of T cells was observed even in cultures depleted of macrophages, suggesting that activation by anti-G(D3) was not dependent on the presence of accessory cells. T cell proliferation was maximum between days 5 and 7 of stimulation and was preceded by expression of interleukin 2 receptors. No stimulation was observed with control antibodies of the identical isotype or with monoclonal antibodies recognizing the gangliosides G(D2) or G(M2). During stimulation by anti-G(D3) monoclonal antibodies, there was an expansion of the G(D3)+ pool of T cells, but depletion of G(D3)+ T cells prior to stimulation abrogated the response. Proliferation induced by binding to G(D3) could be augmented by exogenous interleukin 2 and phytohemagglutinin. Anti-CD3 (T3) monoclonal antibodies had little or no effect. These results demonstrate that binding to G(D3) on the surface of T cells can elicit signals for T cell proliferation. |
| Keywords: | flow cytometry; lymphocyte proliferation; t lymphocyte; t-lymphocytes; interleukin 2; in vitro study; monoclonal antibody; lymphocyte activation; antibodies, monoclonal; thymus gland; carrier proteins; macrophages; interleukin-2; receptors, immunologic; gangliosides; ganglioside; receptors, interleukin-2; antigens, cd2; humans; human; blood and hemopoietic system |
| Journal Title: | Journal of Immunology |
| Volume: | 139 |
| Issue: | 6 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1987-09-15 |
| Start Page: | 1763 |
| End Page: | 1771 |
| Language: | English |
| PUBMED: | 2887619 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
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