Human melanoma cell lines deficient in G(D3) ganglioside expression exhibit altered growth and tumorigenic characteristics Journal Article
| Authors: | Nakano, J.; Mohan Raj, B. K.; Asagami, C.; Lloyd, K. O. |
| Article Title: | Human melanoma cell lines deficient in G(D3) ganglioside expression exhibit altered growth and tumorigenic characteristics |
| Abstract: | We have selected G(D3)-deficient human melanoma cell lines, in order to investigate the function of G(D3) ganglioside. This was done by treating SK-MEL-28 cells with anti-G(D3) antibody (R24) and rabbit complement and subsequent subcloning of the surviving cells, resulting in the derivation of two cell lines deficient in the cell surface expression of G(D3). Neither cell line (designated SK-MEL-28-N1 and SK-MEL-28-N2) had detectable cell surface expression of G(D3) as analyzed with monoclonal antibody R24, and no G(D3) was detectable in either cell line by glycolipid isolation, thin-layer chromatography, or resorcinol-HCl spray, but thin-layer chromatography immunostaining with monoclonal antibody R24 showed the presence of low amounts of G(D3) in both N1 and N2 (1/40 of the amount in the parent cell line in N1 and 1/500 in N2). In SK-MEL-28-N1, the residual G(D3) was shown by immunofluorescence assays on permeabilized cells to be present in discrete intracellular organelles, suggesting that these cells have a defect in the transport of G(D3) as well as in its synthesis. Both SK-MEL-28-N1 and -N2 had an increase in detectable G(D3) expression. The mutant cell lines had altered cell morphology in comparison to the parent cell line and both had slower growth rates in vivo and lower tumorgenicity in nu/nu mice. These results indicate that G(D3) ganglioside plays an important role in proliferation and growth of melanoma cells. |
| Keywords: | controlled study; human cell; nonhuman; animal cell; mouse; melanoma; carcinogenesis; ganglioside gd3; glycolipids; glycosyltransferase; human; priority journal; article; cell pigmentation |
| Journal Title: | Journal of Investigative Dermatology |
| Volume: | 107 |
| Issue: | 4 |
| ISSN: | 0022-202X |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1996-10-01 |
| Start Page: | 543 |
| End Page: | 548 |
| Language: | English |
| DOI: | 10.1111/1523-1747.ep12582802 |
| PUBMED: | 8823358 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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