SNAREs can promote complete fusion and hemifusion as alternative outcomes Journal Article
| Authors: | Giraudo, C. G.; Hu, C.; You, D.; Slovic, A. M.; Mosharov, E. V.; Sulzer, D.; Melia, T. J.; Rothman, J. E. |
| Article Title: | SNAREs can promote complete fusion and hemifusion as alternative outcomes |
| Abstract: | Using a cell fusion assay, we show here that in addition to complete fusion SNAREs also promote hemifusion as an alternative outcome. Approximately 65% of events resulted in full fusion, and the remaining 35% in hemifusion; of those, approximately two thirds were permanent and approximately one third were reversible. We predict that this relatively close balance among outcomes could be tipped by binding of regulatory proteins to the SNAREs, allowing for dynamic physiological regulation between full fusion and reversible kiss-and-run-like events. © The Rockefeller University Press. |
| Keywords: | controlled study; nonhuman; protein conformation; animal cell; animals; mice; protein binding; prediction; physiology; recombinant proteins; cell membrane; 3t3 cells; regulator protein; cell fusion; cho cells; cricetinae; cricetulus; vesicular transport proteins; membrane fusion; snare protein; snare proteins |
| Journal Title: | Journal of Cell Biology |
| Volume: | 170 |
| Issue: | 2 |
| ISSN: | 0021-9525 |
| Publisher: | Rockefeller University Press |
| Date Published: | 2005-07-18 |
| Start Page: | 249 |
| End Page: | 260 |
| Language: | English |
| DOI: | 10.1083/jcb.200501093 |
| PUBMED: | 16027221 |
| PROVIDER: | scopus |
| PMCID: | PMC2171417 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 81" - "Export Date: 24 October 2012" - "CODEN: JCLBA" - "Source: Scopus" |
