Structure of the angiopoietin-2 receptor binding domain and identification of surfaces involved in Tie2 recognition Journal Article
| Authors: | Barton, W. A.; Tzvetkova, D.; Nikolov, D. B. |
| Article Title: | Structure of the angiopoietin-2 receptor binding domain and identification of surfaces involved in Tie2 recognition |
| Abstract: | The angiopoietins comprise a small class of secreted glycoproteins that play crucial roles in the maturation and maintenance of the mammalian vascular and lymphatic systems. They exert their effects through a member of the tyrosine kinase receptor family, Tie2. Angiopoietin/Tie2 signaling is unique among tyrosine kinase receptor-ligand systems in that distinct angiopoietin ligands, although highly homologous, can function as agonists or antagonists in a context-dependent manner. In an effort to understand this molecular dichotomy, we have crystallized and determined the 2.4 Å crystal structure of the Angiopoietin-2 (Ang2) receptor binding region. The structure reveals a fibrinogen fold with a unique C-terminal P domain. Conservation analysis and structure-based mutagenesis identify a groove on the Ang2 molecular surface that mediates receptor recognition. ©2005 Elsevier Ltd All rights reserved. |
| Keywords: | controlled study; human cell; mutation; protein domain; mammalia; carboxy terminal sequence; calcium; amino acid sequence; molecular sequence data; molecular recognition; crystal structure; protein structure, tertiary; binding sites; protein structure; crystallization; angiopoietin receptor; receptor, tie-2; mutagenesis; crystallography |
| Journal Title: | Structure |
| Volume: | 13 |
| Issue: | 5 |
| ISSN: | 0969-2126 |
| Publisher: | Cell Press |
| Date Published: | 2005-05-01 |
| Start Page: | 825 |
| End Page: | 832 |
| Language: | English |
| DOI: | 10.1016/j.str.2005.03.009 |
| PUBMED: | 15893672 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 18" - "Export Date: 24 October 2012" - "CODEN: STRUE" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work