Surveillance of different recombination defects in mouse spermatocytes yields distinct responses despite elimination at an identical developmental stage Journal Article
| Authors: | Barchi, M.; Mahadevaiah, S.; Di Giacomo, M.; Baudat, F.; De Rooij, D. G.; Burgoyne, P. S.; Jasin, M.; Keeney, S. |
| Article Title: | Surveillance of different recombination defects in mouse spermatocytes yields distinct responses despite elimination at an identical developmental stage |
| Abstract: | Fundamentally different recombination defects cause apoptosis of mouse spermatocytes at the same stage in development, stage IV of the seminiferous epithelium cycle, equivalent to mid-pachynema in normal males. To understand the cellular response(s) that triggers apoptosis, we examined markers of spermatocyte development in mice with different recombination defects. In Spo11-/- mutants, which lack the double-strand breaks (DSBs) that initiate recombination, spermatocytes express markers of early to mid-pachynema, forming chromatin domains that contain sex body-associated proteins but that rarely encompass the sex chromosomes. Dmc1-/- spermatocytes, impaired in DSB repair, appear to arrest at or about late zygonema. Epistasis analysis reveals that this earlier arrest is a response to unrepaired DSBs, and cytological analysis implicates the BRCT-containing checkpoint protein TOPBP1. Atm-/- spermatocytes show similarities to Dmc1-/- spermatocytes, suggesting that ATM promotes meiotic DSB repair. Msh5 -/- mutants display a set of characteristics distinct from these other mutants. Thus, despite equivalent stages of spermatocyte elimination, different recombination-defective mutants manifest distinct responses, providing insight into surveillance mechanisms in male meiosis. Copyright © 2005, American Society for Microbiology. All Rights Reserved. |
| Keywords: | unclassified drug; mutation; nonhuman; animal cell; mouse; spermatocyte; cytology; meiosis; animals; mice; spermatocytes; animal tissue; dna repair; apoptosis; protein; mice, inbred c57bl; time factors; mice, transgenic; dna strand breakage; molecular marker; gene expression regulation, developmental; double stranded dna; genetic recombination; chromatin; recombination, genetic; atm protein; microscopy, fluorescence; models, genetic; chromosome protein; testis; developmental stage; mutant; spo11 protein; fluorescent antibody technique, indirect; epistasis; sex chromosome; epistasis, genetic; seminiferous tubule; protein dmc1; protein topbp1 |
| Journal Title: | Molecular and Cellular Biology |
| Volume: | 25 |
| Issue: | 16 |
| ISSN: | 0270-7306 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2005-08-01 |
| Start Page: | 7203 |
| End Page: | 7215 |
| Language: | English |
| DOI: | 10.1128/mcb.25.16.7203-7215.2005 |
| PUBMED: | 16055729 |
| PROVIDER: | scopus |
| PMCID: | PMC1190256 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 68" - "Export Date: 24 October 2012" - "CODEN: MCEBD" - "Source: Scopus" |
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