CD4+ T cell responses to SSX-4 in melanoma patients Journal Article
| Authors: | Ayyoub, M.; Merlo, A.; Hesdorffer, C. S.; Rimoldi, D.; Speiser, D.; Cerottini, J. C.; Chen, Y. T.; Old, L. J.; Stevanovic, S.; Valmori, D. |
| Article Title: | CD4+ T cell responses to SSX-4 in melanoma patients |
| Abstract: | Genes of the synovial sarcoma X breakpoint (SSX) family are expressed in different human tumors, including melanomas, but not in adult somatic tissues. Because of their specific expression at the tumor site, SSX-encoded Ags are potential targets for anticancer immunotherapy. In this study, we have analyzed CD4+ T cell responses directed against the Ag encoded by SSX-4. Upon in vitro stimulation of PBMC from four melanoma patients bearing Ag-expressing tumors with a pool of long peptides spanning the protein sequence, we detected and isolated SSX-4-specific CD4+ T cells recognizing several distinct antigenic sequences, mostly restricted by frequently expressed HLA class II alleles. The majority of the identified sequences were located within the Krüppel-associated box domain in the N-terminal region of the protein, indicating a high potential immunogenicity of this region. Together our data document the existence of CD4+ T cells specific for multiple SSX-4 derived sequences in circulating lymphocytes from melanoma patients and encourage further studies to assess the impact of SSX-4-specific T cell responses on disease evolution in cancer patients. Copyright © 2005 by The American Association of Immunologists, Inc. |
| Keywords: | adult; controlled study; protein expression; unclassified drug; human cell; major clinical study; t lymphocyte; allele; melanoma; neoplasm proteins; protein; alleles; in vitro study; cell line, tumor; cell specificity; amino acid sequence; molecular sequence data; antigen; amino terminal sequence; antigens, neoplasm; immunogenicity; hla antigen class 2; cd4-positive t-lymphocytes; epitope mapping; cell isolation; protein structure, tertiary; cell stimulation; histocompatibility antigens class ii; genes, mhc class ii; repressor proteins; peripheral blood mononuclear cell; peptide derivative; epitopes; synovial sarcoma x 4 protein |
| Journal Title: | Journal of Immunology |
| Volume: | 174 |
| Issue: | 8 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 2005-04-15 |
| Start Page: | 5092 |
| End Page: | 5099 |
| Language: | English |
| PUBMED: | 15814740 |
| PROVIDER: | scopus |
| DOI: | 10.4049/jimmunol.174.8.5092 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 15" - "Export Date: 24 October 2012" - "CODEN: JOIMA" - "Source: Scopus" |
